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| 基底拉伸应变对小鼠成骨细胞Runx2表达的影响 |
| The effect of substrate-stretching strain on the expression of Runx2 in mouse osteoblasts |
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| DOI： |
| 中文关键词: 基底拉伸应变 成骨细胞 |
| 英文关键词:Substrate-streching strain Runx2 Osteoblasts |
| 基金项目:国家自然科学基金重点资助项目(10832012) |
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| 中文摘要: |
| 目的 研究基底拉伸应变对小鼠成骨细胞系MC3T3-E1细胞Runx2表达的影响.方法 实验随机分为0 με、1000 με、1500 με、2000 με、2500 με、5000 με六组,采用卫生装备研究所自行研制的细胞基底应变加载装置对细胞进行加载,Western blot检测不同应变对成骨细胞Runx2蛋白表达的影响.此外采用Real-time PCR检测0 με、2500 με、5000 με三种强度应变对成骨细胞Runx2 mRNA表达的影响.结果 Western blot结果显示:1500 με、2000 με、2500 με组与0 με组相比Runx2蛋白表达显著增强(P＜0.01);5000με组与0 με组相比Runx2蛋白表达显著降低(P＜0.01);Real-time PCR结果显示:2500 με组与0 με组相比Runx2 mRNA表达显著降低(P＜0.01);5000 με组与0 με组相比Runx2 mRNA表达显著增加(P＜0.01).结论 ①生理强度的拉伸应变可显著增加MC3T3-E1细胞Runx2蛋白的表达,并且呈剂量依赖性,超生理强度5000 με显著降低Runx2蛋白表达.②同样强度的拉伸应变刺激下,Runx2基因表达与蛋白表达并不一致. |
| 英文摘要: |
| Abstract：Objective To study the effect of substrate stretching strain on the expression of Runx-2 by mouse MC3T3-El osteoblasts．Methods Six experimental groups were randomized divided to 0με，1000με，1500με，2000με，2500με，and 5000μεgroups．The ceHs were loaded with substrate-stretching strain loading system．Runx-2 protein expression in cells with different strain groups was detected using western blotting．Runx-2 mRNA expression in ceHs subjected to Oμε，2500με，and 5000μεsubstratestretching strain was detected using real time PCR．Results Western blotting results showed that the expressions of Runx-2 protein were significantly increased in 1500με，2000με，and 2500μεgroups，and decreased in 5000μεgroup，comparing to that in Oμεgroup(P<0．01)．Real-time PCR results showed that the expression of Runx-2 mRNA was significantly decreased in 2500μεgroup，and increased in 5000μεgroup，comparing to that in 0μεgroup(P<0．01)．Conclusion (1)Expression of Runx2 protein in MC3T3-El cells could be up—regulated under physiological strain and the regulation was magnitude dependent．The expression was down regulated when the strain magnitude was beyond physiological load at 5000με．(2)Expression of Runx2 mRNA was not consistent with the expression of the protein under the same strain magnitude． |
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