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| 镉诱导大鼠骨组织及成骨细胞金属硫蛋白基因表达 |
| Cadmium induced expression of metallothionein mRNA in the bone tissue and in osteoblasts in rats |
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| DOI： |
| 中文关键词: 镉 大鼠 成骨细胞 金属硫蛋白 |
| 英文关键词:admium Rat Osteoblast Metallothionein |
| 基金项目:欧盟合作课题(FOOD．CT-2006-016253) |
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| 中文摘要: |
| 目的 探讨镉(Cd)对大鼠骨组织及体外培养成骨细胞中金属硫蛋白mRNA表达的影响.方法 应用皮下注射的方法对Sprague-Dawley雄性大鼠进行连续Cd染毒(0～1.5 mg/kg bw)12周,5d/w,收集血样、尿样及骨组织,分别测定体内镉负荷;应用逆转录-聚合酶链反应(RT-PCR)方法检测骨组织中金属硫蛋白(MT)基因表达;同时,应用混合酶消化法分离大鼠颅盖骨成骨细胞,进行原代培养,并以不同浓度的Cd(0～2.0 μmol/L)作用24 h以及1.0 μmol/L Cd作用不同时间(24～72 h)后,应用RT-PCR,观察成骨细胞MT mRNA表达的变化.结果 大鼠镉染毒后体内镉负荷显著增加,呈明显剂量-效应关系;镉染毒能够诱导大鼠骨组织MT基因表达,各剂量染毒大鼠骨组织MT基因表达均明显高于对照组,P＜0.05.体外实验结果同样表明,镉作用能诱导成骨细胞MT表达,并随着染镉剂量的增加,MT基因表达也明显增高,特别是在0.5 μmol/L和2.0 μmol/L组,和对照组相比差异有显著意义(P＜0.01);1.0 μmol/L CdCl2作用不同时间后均能诱导成骨细胞MT表达,但表达量并不随锅作用时间而出现明显改变.结论 镉能诱导骨组织及体外培养成骨细胞MT表达,MT可能镉在致骨损伤中有一定保护作用. |
| 英文摘要: |
| Abstract：Objective To investigate the effect of cadmium(Cd)on meudlothionein (MT)mRNA expression in the bone tissue and in osteoblasts in rats．Methods Sprague-Dawley(SD)male rats were injected with Cd subcutaneously at the doses of 0,0.1,O.5,and 1.5 mg／kg,5 times per week，for 12 weeks．Blood，24h urine，and bone tissue were collected for examining Cd loading．MT mRNA expression in the bone Was determined using reverse transcription polymerase chain reaction(RT-PCR).Meanwhile，osteoblasts were harvested from the rat calvaria using sequential enzyme digestion method.Cells were cultured and treated with different concentrations of Cd(0-2.0μmol／L)for 24h or different times (24-72 h)at 1.Oμmol／L.MT mRNA expression in osteoblasts Was determined using RT-PCR．Results The Cd concentration in the rat Was significantly increased after Cd loading．The increase Was dose-dependent．Cd injeetion induced MT expression in the bone tissue．MT expression in rats with Cd injection of all doses Was significantly higher than that in the control (P<0.05).In vitro experiment demonstrated that Cd induced MT expression in osteoblasts．The increase of MT expression Was dose-dependent，espeeially at dose of 0.5 and 2.0μmol／L of Cd(P<0.01).The increase Was significantly different compared with the control．Cd (1．Olμmol／L)induced MT expression in osteoblasts，but the expression level Was not time-dependent．Conchsion Cd could induce MT expression in the bone tissue and in cultured osteoblasts in vitro．MT may play a proteetive role in Cd—induced osteotoxicity． |
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