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| 去势大鼠股骨组织中Wntl0b的表达 |
| WntlOb expression in the femur of castrated rats |
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| DOI： |
| 中文关键词: 大鼠 去势 骨质疏松 WntlOb |
| 英文关键词:Rats Castrating Osteoporosis Wntl0b |
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| 中文摘要: |
| 目的 观测去势大鼠骨质疏松模型中股骨组织Wntl0b的表达。方法 20只SD雌性大鼠随机分为去势组和对照组两组。大鼠切除双侧卵巢去势诱发骨质疏松症。术后3个月腹主静脉取血，用放射免疫法测定各组大鼠血清E2水平。从股骨中提取总RNA和蛋白，分别采用RT-PCR和免疫印迹的方法检测wntlOb在mRNA和蛋白表达水平上的变化。结果 去势组大鼠血清E2含量为10.228±4.094 pmoL/L；对照组血清E2含量为21.975±3.021 pmo/L。经RT-PCR和免疫印迹检测发现，与对照组相比，去势大鼠Wntl0b的mRNA和蛋白水平表达量均降低。结论 去势大鼠骨质疏松模型体内股骨组织中Wntl0b的mRNA和蛋白表达水平均下降，以上结果提示Wntl0b可能在大鼠去势诱发骨质疏松模型中通过调节成骨等过程参与了骨质疏松的发展过程。 |
| 英文摘要: |
| Abstract：0bjective To observe and measure the expression level of Wntl0b in the femur of ovariectomized osteoporosis rat model．Methods Twenty female SD rats were randomly divided into 2 groups：the ovariectomized group and the model control group.The rats were ovariectomized to induce osteoporosis.After 3 months，blood was withdrawn from the cardinal vein and serum E2 was using immunoradiological method．Total RNA and protein were collected from the femur．Expression of Wntl0b mRNA and protein was detected using RT-PCR or Western bloting．Results The concentration of serum E2 was 10.228±4.094 pmol/L in ovafieetomized group．and 21.975±3.021 pmol/L in teh model control group，respectively．The mRNA and protein expression of Wntl0b in rats of ovafiectomized group was lower than those of model control group．Conclusion Both mRNA and protein expression of WntlOb were lower in rats of ovariectomized group，indicating that WntlOb might play an important role in ovariectomized rats to induce the development of osteoporosis through regulating bone formation |
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