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| rhPTHl-34对成骨细胞增殖及BMP-7、BMP-9基因表达的影响 |
| Effect of recombinant human PTH 1-34 on the proliferation of osteoblasts and the gene expression of bone morphogenetic protein-7 and bone morphogenetic protein-9 |
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| DOI： |
| 中文关键词: 重组人甲状旁腺激素1-34(rhPTHl-34) 成骨细胞 增殖 BMP-7 BMP-9 |
| 英文关键词:rhPTHl-34 Osteoblasts Proliferation BMP-7 BMP-9 |
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| 中文摘要: |
| 目的 研究重组人甲状旁腺激素1-34(rhPTHI-34)对成骨细胞增殖及BMP-7、BMP-9基因表达的影响。方法 通过不同剂量的重组人甲状旁腺素(rhPTHl-34)(0、10-11、10-10、lO-9、lO-8、10-7mol/L)间歇性(24 h/周期，前12 h rhPTHl-34干预)刺激体外培养的成骨细胞，用噻唑蓝(MTT)法检测细胞的增殖能力，RT-PCR法检测成骨细胞BMP-7、BMP-9基因的表达。结果 间歇性小剂量rhPTHI．34可明显促进成骨细胞的增殖能力及增强BMP-7、BMP-9基因的表达。结论 间歇性小剂量rhPTHl_34可促进成骨细胞增殖，可能与BMP-7、BMP-9基因表达相关。 |
| 英文摘要: |
| Abstract：Objective To study the effect of recombinant human parathyroid hormone (rhPTHl-34)on the proliferation of osteoblasts and the gene expression of bone morphogenetic protein-7(BMP-7)and bone morphogenetic protein-9(BMP-9)． Methods Cultured osteoblasts were treated with different concentrations of rhPTHl．34(0，10-11，10-10，lO-9，10-8，and 10-7mol/L)，at the first 12h of a 24h interval．Cell proliferation was assessed using MTT colofimetric assay．The expression of BMP-7 and BMP-9 genes was examined with semi·quantitative RT-PCR．Results rhPTHl·34 promoted osteoblast proliferation (P<0.01)and increased the expression of BMP-7 and BMP-9 genes with low dose and intermittent administration．Conclusion LoW dose and intermittent administration of rhPTHl-34 indueed osteoblast proliferation，probably in association with the expression of BMP-7 and BMP-9． |
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