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| 一种定量药理学方法研究护骨胶囊的药效并分析各味药对药效的协同和拮抗作用 |
| Study the efficacy of the Hugu capsule and Analysis of various herbs on the efficacy of synergism andantagonism by a quantitative pharmacological method |
| 投稿时间：2012-03-12 |
| DOI： |
| 中文关键词: HG 成骨细胞 分化 增殖 |
| 英文关键词:Hugu capsules Osteoblast(0b) differentiation proliferation |
| 基金项目: |
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| 摘要点击次数: 1096 |
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| 中文摘要: |
| ：目的以成骨细胞增殖和分化为指标，通过正交模拟法与非线性混合效应模型(NONMEM)
(DAS3．0软件)分析护骨胶囊(HG)各昧药对药效的协同和拮抗作用，并对该方不同组合的复方进行
药效模拟。方法将HG 10个组分按照设计的正交表分为13个组，血清药理学实验获取13个组的
含药血清，CCK一8法及AKP法检测各组血清对成骨细胞(Ob)增殖和分化的影响。DAS3．0软件处理
数据，分析各味药对药效的协同和拮抗作用，并对复方药效进行模拟。结果(1)以0b分化为指标，
淫羊藿、山药、巴戟天、熟地黄、杜仲对成骨细胞分化有促进作用，骨碎补、制首乌无作用，龟甲、当归、
续断对Ob分化有抑制作用；以Ob增殖为指标，淫羊霍、骨碎补、巴戟天、山药、龟甲、当归、熟地黄、续
断对Ob增殖有促进作用，杜仲及制首乌对ob增殖无作用。(2)以两种指标进行观察，DAS3．0软件
处理不同组合的复方进行药效模拟发现，分化药效预测最大值为0．2263，且共有6个组分化药效预
测值大于0．2，原方排第二；增殖药效预测值最大为1．1620．为原方，另外还有4个组增殖药效大于
1．0；此外，将两个结果综合发现，有三个模拟组方同时满足分化大于0．2和增殖大于I．0。结论利
用0b增殖和分化为指标，结合定量药理学方法综合分析HG，可提供丰富的组方信息，为HG的研究
和开发提供了理论基础。 |
| 英文摘要: |
| Abstract：Objective With proliferation and differentiation of Osteoblasts∞the index，The orthogonal
simulation method and NONMEM method were used to Study the efficacy of the Hugu capsule and Analysis of
various herbs on the efficacy of synergism and antagonism．Method HG 10 components were divided into
13 groups．the drug serum of 13 groups were gotten by the serum pharmacological experiments，the method of
AKP and CCK·8 were adopted to detect proliferation and differentiation of osteoblast and pharmacodynamic
simulation of compound were analysis by DAS3．0 software．Result the differentiation of Ob“a index．the
effect were promoted by epimedium，yam，Mofinda officinalis，prepared rehmannia root and Eucommia
ulmoides．Drynaria and Polygonum muhiflorum were non-pharmaeodynamic．The effect Wag inhibited byTurtleback。angelica and Himalayan Teasel Root．The proliferation of Ob∞a index，the effect was promoted
by epimedium，drynaria，Morinda officinalis，yam．turtleback．angelica，prepared rehmannia root and
Himalayan Teasel Root．Eucommia ulmoides and Polygonum muhiflorum were non-pharmacodynamie．(2)
With proliferation and differentiation of Osteoblasts as the index．and date were analysised by DAS3．0
software．The results of pharmacndynamics of simulation compositions showed 6 groups differentiation
efficacy predictive value greaterthan0，2．themaximum value of efficacy predictedis 0．2263．the HGwasthe
second．5 groups proliferation efficacy predictive value greater than 1．0，the HG was the first，and value of
efficacy predicted is 1．1620；In addition，there are three simulation group meet to differentiation is greater
than 0．2 and the proliferation of greater than 1．0．Conclusion Quantitative pharmacology methods were
analysis of HG with proliferation and differentiation of Osteoblasts∞the index．The method can provide rich
formula information for HG，and provide a theoretical basis for reSeal℃h and development of the HG． |
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