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| 骨碎补总黄酮防治绝经后骨质疏松症的实验研究 |
| Experimental study of drynaria foMunei J．SM／or the prevention of postmenopausal osteoporosis |
| 投稿时间：2012-01-13 |
| DOI： |
| 中文关键词: 骨碎补总黄酮 去卵巢大鼠 组织蛋白酶K 生物力学 |
| 英文关键词:Key words：Drynaria fortunei J．SM Ovariectomized rats Cathepsin K Biomechanics |
| 基金项目: |
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| 中文摘要: |
| 目的探讨骨碎补总黄酮防治骨质疏松的机理，为补肾中药有效成分治疗骨质疏松症提供实
验依据。方法选取3月龄雌性SD大鼠72只，随机分为6组：给药组切除双侧卵巢给予骨碎补总黄
酮灌胃，分为：高剂量组，中剂量组，低剂量组；对照组，切除双侧卵巢给予雌激素灌胃；假手术组，开腹
后切除一小块卵巢周围脂肪给予常规喂饲；夺白组不做处理常规喂饲。分别在第0、3、6个月处死每
次每组4只。取左胫骨近端干骺端标本，运用荧光定量PCR方法测定Cathepsin K mRNA表达量，运
用三点弯曲法测胫骨干最大弯曲载荷。采用SPSS软件进行数据统计分析。结果给药组与对照组胫
骨干骺端Cathepsin K mRNA表达量相比，有统计学意义(P<0．05)。给药组与假手术，空白组均有显
著性差异(P(0．01)；胫骨干最大弯曲载荷显示：给药组与对照组有统计学意义(P<0．05)，给药组与
假手术，空白组均有显著性差异(P<0．01)。结论骨碎补总黄酮可以降低胫骨干骺端Cathepsin K
mRNA的表达量，提高胫骨干最大弯曲载荷。此可能为骨碎补总黄酮治疗骨质疏松症的重要机制。 |
| 英文摘要: |
| Objective To explore the mechanism of drynaria fortunei J．SM for the prevention of
postmcnopausal osteoporosis and to provide experimental evidence of the kidney-supplementing drynaria
fnRune J．SM for the treatment of esteoporosis．Methods A total of 72 3-month·old female rats were
selected and randomly divided into 6 groups．The rats in the drag group were administered with drynafia
fortunei J．SM after bilateral ovariectomy and divided into high·dose group，middle—dose group，and low-dose
group，The rats in the control group were administered with estrogen after bilateral evarieetomy．Rats in the
sham surgery group were administered with routine feed after the resection of a little fat near the ovary．Rats
in the blank group were not given any treatment．Four rats in every group were killed in 0，3，and 6 months．
The proximal metaphysics of the left tibia of rats were sectioned．The expression of cathepsin K mRNA was
measured using fluorescence quantitative PeR．Bending load of the tibia was measured using three point
bending test．The statistical analysis was performed with SPSS 13．0 software．Results The expression of
eathepsin K mRNA between drag group and control group was significantly different(P<0．05)．The
expression of cathepsin K mRNA between drug group and sham surgery group，and blank group was also
significantly different(P<0．01)．The bending load of the tibia between drug group and control group was
significantly different(P<0．05)．The bending load of the tibia between drug group and sham surgery
group，and blank group was also significantly different(P<0．01)．Conclusion Drynaria fortune J．SM
can decrease the expression of cathepsin K mRNA of metaphysics of the tibia and increase the bending load
of the tibia．This may be all important mechanism of drynafia fortunei J．SM for the treatment of osteoporosis． |
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