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| 不同浓度铁离子对成骨细胞铁离子通道蛋白的影响 |
| The effect of iron with different concentrations on the iron channel protein in osteoblasts |
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| DOI：10.3969/j.issn.1006.7108.2012.08.004 |
| 中文关键词: 人成骨细胞 铁离子 膜转铁蛋白 转铁蛋白受体 二价金属转运蛋白 |
| 英文关键词:Osteoblasts Iron ion Ferroportin Transferring receptor Divalent metal transporter 1 |
| 基金项目:教育部博士学科点专项科研基金（3201 110020 )、江苏省领军人才基金 |
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| 中文摘要: |
| 目的 铁过载与骨代谢相关，在人成骨细胞（hFOB1. 19 )中，膜转铁蛋白（FPN1 )、转铁蛋白受体（TfR)和二价金属转运蛋白1( DMT1 )是细胞内铁离子进入和排除的重要通道蛋白；本研究用不同浓度铁离子干预成骨细胞培养，观察成骨细胞铁离子通道蛋白基因表达变化和相互联系，了解铁过载对相关通道蛋白的影响意义。方法 人成骨细胞在34℃下进行体外培养，以不同浓度枸橡酸铁铵 FAC( 50μmol/L、100μmol/L、200μmol/L )干预成骨细胞培养，48小时后收集细胞，按不同干预组抽提总RNA，采用半定量RT-PCR法检测成骨细胞中TfR、DMT1、FPN1 mRNA的表达。结果 ①RT- PCR检测显示不同浓度FAC干预成骨细胞后，各组FPN1、TfR、DMT1 mRNA均有表达；②不同浓度组 FPN1、TfR、DMT1 mRNA表达光密度比值不同，组间密度比值比较存在统计学意义（P<0.05 )；③培养环境铁离子浓度增高可以使得FPN1的mRNA表达上调，而TfR、DMT1的mRNA表达下调。结论 成骨细胞的铁通道蛋白受环境铁离子影响，在一定范围内随着细胞外铁离子浓度增加，细胞膜铁离子进入通道功能下调、排除通道功能上调，说明铁过载对成骨细胞内铁离子水平有明显影响。 |
| 英文摘要: |
| Objective Iron overload was associated with osteoporosis. The ferroportin ( FPN1 )， transferring receptor (TfR ), and divalent metal transporter 1 (DM T1 ) were the important channel proteins of the iron ions in osteoblasts ( hFOB1. 19 ). This study aimed to culture osteoblast with different does of iron ions，and to observe gene expression variation of iron ion channel proteins, and to acknowledge the effect of iron overload on the relevant channel proteins. Methods The osteoblasts were cultured at 34℃ in vitro with different concentrations of FAC ( 50μmol/L，100μmol/L，and 200μmol/L，respectively ) for 48 hours.The total RNA in different intervention groups was isolated and extracted. Expressions of TfR,DMT1 and FPN1 mRNA were detected with semi-quantitative RT-PCR. Results The resulL of RT-PCR showed that the mRNA of TfR，DMT1,and FPN1 was expressed after the intervention of FAC with different concentrations.The density ratios of TfR,DMT1, and FPN1 mRNA were different among the different FAC concentration groups,and the differences were statistically significant (P<0.05 ). The expression of FPN1 mRNA increased with the increasing of iron concentration. However the expression of TfR and DMT1 mRNA decreased with the increasing of iron concentration. Conclusion The iron channel proteins of osteoblasts can be affected by the iron ions. Along with the increasing of iron ions concentration in a certain range, the function of iron into channel can be down-regulated and the function of iron out the channel can be up regulated. The effect of iron overload on the level of iron in osteoblasts is significant. |
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