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| 中医不同方法对骨质疏松症大鼠骨骼、 骨骼肌I型胶原蛋白表达影响的实验研究 |
| Study of the effect of different Chinese medicine treatments onproteinexpressionof COL I inbone tissue of osteoporotic rats |
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| DOI： |
| 中文关键词: 骨质疏松症 骨骼肌 I型胶原 |
| 英文关键词:Osteoporosis Skeletal muscle Collagen I |
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| 中文摘要: |
| 目的观察骨质疏松症大鼠骨骼、骨骼肌I型胶原蛋白表达变化的影响，探讨中医防治骨质疏 松症的作用机制。方法将120只雌雄各半的大鼠随机分为正常对照组、模型对照组、补肾中药组、 健脾中药组、活血化瘀中药组和骨疏康中药组6个组。采用Western blot方法测定大鼠骨骼、骨骼肌 I型胶原蛋白表达。结果①各组大鼠骨组织均可检测到I型胶原的蛋白表达。与正常组比较，各 组大鼠骨组织I型胶原的蛋白表达均明显下降（！<0.01);与模空组比较，各治疗组大鼠骨组织I型 胶原的蛋白表达均明显升高，其中补肾组、骨疏康组升高趋势最显著（P<0. 01 )。②各组大鼠骨骼肌 均可检测到I型胶原的蛋白表达。与正常组比较，各组大鼠骨骼肌I型胶原的蛋白表达均明显下降 (P<0.01)；与模空组比较，各治疗组大鼠骨骼肌I型胶原的蛋白表达均明显升高（!<0.01)，其中补 肾组升高趋势最明显。结论①骨质疏松症的形成，可能与骨骼、骨骼肌I型胶原表达的异常变化有 关。②补肾、健脾方法通过调控骨质疏松症大鼠的骨骼、骨骼肌I型胶原蛋白表达，对骨质疏松症具 有一定的防治作用。 |
| 英文摘要: |
| Objective To explore the mechanism of traditional Chinese herb in treatingosteoporosis by investigating protein expression of collagen I ( COL I) in skeleton and skeletal muscle of osteoporotic rats. Methods One hundred and twenty rats ( male/female = 1 : 1 ) were randomly divided into 6 groups as following ： blank control group， model control group， invigorating the kidney group， invigorating the spleen group， promoting blood flow group， and Gusukang group. The protein expression of COL I in skeleton and skeletal muscle was tested using Western-blotting. Results 1 ) Protein expression of COL I could be detected in the skeleton of all the groups. Compared to blank control group， the expression in all other groups decreased significantly ( P <0.05 ) . Compared to model control group， the expression in all the herbal treating groups elevated significantly，among which the expression in invigorating the kidney group and Gusukang group elevated most significantly ( P < 0. 01 ) . 2 ) Protein expression of COL I could be detected in skeleton muscle of all the groups. Compared to blank control group， results in all other groups decreased significantly ( P < 0. 05 ) . Compared to model control group，expression in all herbal treating groups elevated significantly， among which the expression in invigorating the kidney group elevated most significantly ( P < 0.01). Conclusion 1) The development of osteoporosis may be associated with abnormal expression of COL I in the skeleton and skeletal muscle. 2 ) Treatments of invigorating the kidney and invigorating the spleen show its efficacy to prevent osteoporosis by regulating the protein expression of COL I in the skeleton and skeletal muscle. |
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