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| 糖尿病大鼠骨质疏松发病过程中骨桥蛋白变化的实验研究 |
| The experimental study of the changes of osteopontin during the genesis of osteoporosis in diabetic rats |
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| DOI：10.3969/j.issn.1006-7108.2012.10.003 |
| 中文关键词: 糖尿病 骨质疏松 骨桥蛋白 |
| 英文关键词:Diabetes, Osteoporosis, Osteopontin |
| 基金项目: |
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| 中文摘要: |
| 目的 研究糖尿病大鼠骨质疏松发病过程中骨桥蛋白的表达。方法 24只雄性Wistar大鼠随机分成2组，糖尿病大鼠组12只，空白对照组12只，饲养4月后处死动物，检查骨桥蛋白mRNA水平表达。 结果 糖尿病组骨桥蛋白总RNA提取及RT-PCR、琼脂糖凝胶电泳显示，糖尿病组大鼠在骨质疏松发病过程中，骨桥蛋白在骨组织中高表达，与空白对照组相比，差异具有显著性。 结论 骨桥蛋白在糖尿病大鼠骨组织有显著性改变，推测骨桥蛋白参与调节骨质疏松发病过程。 |
| 英文摘要: |
| Objective To investigate the expression of osteopontin (OPN) during the genesis of osteoporosis in diabetes rats. Methods Twenty-four healthy male Wistar rats were randomly divided into two groups: the diabetic rat group and the blank control group. Each group had 12 rats. All the rats were executed after being raised for 4 months and the expression of OPN mRNA was examined. Results The total RNA extraction, RT-PCR, and agarose gel electrophoresis showed that OPN in diabetic rat group was overexpressed during the genesis of osteoporosis. The difference was significant compared with that in blank control group. Conclusion The level of OPN is significantly changed in the bone tissue of diabetic rats, and we speculate that OPN may participate in the regulation of the genesis of osteoporosis |
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