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| 重组人II型肿瘤坏死因子受体-抗体融合 蛋白对胶原诱导性关节炎大鼠骨质及 骨保护素的影响 |
| The effect of recombinant human type II tumor necrosis factor receptor-antibody fusion protein on bone mineral density and OPG in rats with collagen-induced arthritis |
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| DOI： |
| 中文关键词: ：关节炎，胶原诱导性 受体，肿瘤坏死因子，II型 骨保护素 骨质 |
| 英文关键词: |
| 基金项目:国家自然科学基金（81160376 ) |
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| 中文摘要: |
| 目的研究重组人H型肿瘤坏死因子受体-抗体融合蛋白（rhTNFR:Fc，益赛普）及其他慢作用 药对胶原诱导性关节炎大鼠骨密度及骨保护素的影响，探讨各种方案对骨代谢生化指标、骨质的改 变。方法建立H型胶原诱导的雄性Wislar大鼠CIA模型，随机分为正常对照组、CIA模型对照组、MTX联合强的松龙组、MTX联合羟氯喹和柳氮磺吡啶组、MTX联合益赛普组。分别于第10、21周予 行大鼠四肢关节钼靶拍片及测骨保护素（韵孕郧)。结果 CIA组大鼠第10周出现骨质疏松、关节软 骨、骨破坏、关节间隙狭窄，MTX + pred组大鼠出现骨质疏松，关节腔结构尚完整，MTX + HCQ + 杂杂在 组大鼠出现骨质疏松、关节软骨、骨轻度破坏、关节间隙轻度狭窄，MTX + rhTNFR：Fc组大鼠则仅出 现骨质轻度破坏；第21周时CIA组、MTX + pred组和MTX + HCQ + SSZ组大鼠骨质破坏加重，而MTX + rhTNFR:Fc组大鼠则未见骨质破坏加重。源组血清韵孕郧水平在治疗前均出现降低，第10周时4 组韵孕郧水平均出现降低，但MTX + rhTNFR:Fc组降低较其他3组有统计学意义（孕<0.05 )。21周 时CIA组、MTX + pred组和MTX + HCQ + SSZ组韵孕郧水平均出现进一步降低（孕<0.05 )，但MTX + rhTNFR:Fc组未见明显降低（P >0.05 )。结论rhTNFR:Fc能有效抑制胶原诱导性关节炎大鼠骨质 破坏，其作用机制可能与韵孕郧相关。 |
| 英文摘要: |
| Objective To investigaLe the effect of recomhinant human type II tumor necrosis factor receptor- antibody fusion protein ( rhTNFR :Fc，Etanercept ) and other slxm-acting drugs on bone mineral density (BMD ) and OPG in rats with collagen-induced arthritis ( CIA )， and to explore the changes of bone metaboiic markers and BMD by the treatments. Methods CIA model induced by type II collagen was established in male Wistar rats. The rats were random by divided in to normal control group，C IA model control group，M TX + prednisolone ( pred ) group，M TX + hydroxychloroquine (HCQ ) + sulfasalazine (SSZ ) group，and MTX + rhTNFR :FC group. Joints molybdenum target X-ray of rat limbs and deLection of OPG were perfomied in 10 weeks and 21 weeks，respectively. Results Al the 10th week， rats in CIA group showed osteoporosis，destruction of articular cartilage and bone，and the narrowing of joint space. RaLs in MTX + pred group had osLeoporosis，but their joint cavity strucLure was still inLact. Rats in MTX + HCQ +SSZ group had osteoporosis，miki destruction of articular cartilage and bone， and mild stenosis of joint space. Rats in MTX + rhTNFR ： Fc group only had mUd bone destruction. Al the 21st week, bone destruction |
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