| Objective To evaluate the effect of caffeic acid phenethyl ester (CAPE) on ovariectomized mice with osteoporosis comprehensively by measuring bone biomechanics and bone mineral density ( BMD).Methods A total of 96 12-week C57BL/6J mice were randomly divided into 16 groups (n = 6 in each group). Prevention of osteoporosis using CAPE ： 1 -week sham group ( sham PBS group), ovariectomized group (OVX PBS group) ； 4-week and 7-week sham PBS group, OVX PBS group, OVX CAPE (0.5 mg/ kg) group, and OVX CAPE (1 mg/kg) group. Treatment of osteoporosis using GAPE ： 7-week sham blank group and OVX blank group, and after 4-week intraperitoneal injection, 11 -week sham PBS group, OVX PBS group, OVX CAPE (0.5 mg/kg) group, and OVX CAPE (1 mg/kg) group. All the 96 mice were anesthetized with 0. 3 ml 0. 5% pentobarbital intraperitoneal injection. Blood samples were taken from the heart 5 minutes later. Serum estrogen level was detected using ELISA method. Bilateral humerus and the spine were collected. Three-point bending experiment was performed on the bilateral humerus. BMD of the spine was tested using dual-energy X-ray absorptiometry ( HOLOGIC , USA ) . Results Serum estrogen level in all OVX groups was significantly lower than that in sham group (P <0. 05). In prevention part, BMD in 4-week OVX group was significantly lower than that in sham group, OVX 0.5 mg group, and OVX 1 mg group (P < 0. 05). On biomechanics part, the maximum stress failure index in 1 -week and 4-week OVX |