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| 羟基磷灰石/聚乳酸-聚羟乙酸/骨形态发生蛋白复合生物材料在兔体内生物组织相容性的研究 |
| Study of the histocompatibility of the biomaterial, HA/PLGA/BMP-2 complexes, in rabbits in vivo1 |
| 投稿时间：2012-11-28 |
| DOI： |
| 中文关键词: 羟基磷灰石 聚乳酸-聚羟乙酸 骨形成蛋白 骨组织工程 生物相容性 |
| 英文关键词:HA PLGA BMP-2 Osseous tissue engineering Histocompatibility |
| 基金项目:全军十二五课题（11MS016） |
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| 摘要点击次数: 1162 |
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| 中文摘要: |
| 目的 评价快速成型工艺制作的羟基磷灰石/聚乳酸-聚羟乙酸/骨形态发生蛋白（HA/PLGA/BMP-2）生物材料在新西兰兔体内的生物组织相容性。方法 健康新西兰大白兔20只，随机抽取10只为实验组，10只为对照组。实验组于兔颅顶部植入HA/PLGA/BMP-2生物材料，对照组植入金属钛网。分别于4周、8周、12周通过HE染色、血常规、血生化等评价方法，评价HA/PLGA/BMP-2生物材料在兔体内的组织相容性。结果 实验组植入物周围组织（约1cm）HE染色结果显示:4周见部分中性粒细胞，少量炎性细胞浸润；8周未见中性粒细胞，无炎性细胞浸润；12周未见炎性细胞，周围可见成纤维细胞增生。对照组植入物周围组织（约1cm）HE染色结果显示4周、8周、12周均未见有炎性细胞生成。实验组、对照组肝、肾组织HE染色结果显示均未见有炎性细胞，无新生组织形成。实验组与对照组12周血液学结果比较，差异无显著性意义。结论 HA/PLGA/BMP-2生物材料具有良好的生物相容性，是一种安全、无肝肾毒性的生物材料，在新型骨组织工程材料上有一定的研发潜力。 |
| 英文摘要: |
| Objective To evaluate the histocompatibility of the biomaterial made using rapid prototyping technology, HA/PLGA/BMP-2 complexes, in New Zealand rabbits in vivo. Methods Twenty New Zealand rabbits were randomly divided into 2 groups, 10 rabbits in experimental group and the other 10 rabbits in control group. Rabbits in the experimental group were implanted with HA/PLGA/BMP-2 complexes into muscles of the skull. Meanwhile, titanium cages were implanted into the same position of rabbits in control group. The histocompatibility of HA/PLGA/BMP-2 complexes in rabbits in vivo was evaluated using HE staining and tests of blood routine and biochemistry at the 4th, 8th, and 12th week, respectively. Results HE staining results of the tissue around implantation (approx. 1cm) in experimental group indicated that some neutrophilic granulocytes and few inflammatory cells infiltrated at the 4th week. No neutrophilic granulocytes and inflammatory cells were observed at the 8th week. And no neutrophilic granulocyte but only fibroblast hyperplasia was observed at the 12th week. No inflammatory cell was observed in HE staining in control group at the 4th, 8th, and 12th week, respectively. The HE staining results of the liver and kidney in both groups showed no inflammatory cells or new tissue formation. The results of blood test between two groups at the 12th week showed no significant difference. Conclusion HA/PLGA/BMP-2 has good histocompatibility, with safe characteristics and no liver and renal toxicity. It has great potential in the osseous tissue engineering research. |
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