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| 水通道蛋白1在不同程度退变腰椎间盘中的表达及意义 |
| The expression of aquaporin-1 in various degenerative human intervertebral discs and its significance |
| 投稿时间：2012-10-24 |
| DOI： |
| 中文关键词: 水通道蛋白 椎间盘退变 髓核 纤维环 |
| 英文关键词:Aquaporin-1 Intervertebral disk degeneration Nucleus Fiber ring |
| 基金项目:桂林市科学研究与技术开发计划项目（20120407-2） |
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| 中文摘要: |
| 目的 观察水通道蛋白1在退变腰椎间盘组织中的表达变化,研究其与椎间盘退变的关系。方法 实验组取材于经手术治疗腰椎间盘突出症患者椎间盘标本30例，突出型、脱出型、游离型各10例；对照组取材于腰椎损伤致椎体骨折患者切除椎间盘10例。应用免疫组织化学法检测水通道蛋白1在椎间盘组织中的表达。结果 （1）苏木精-伊红染色显示，对照组椎间盘组织仍保持着致密的板层结构，实验组椎间盘组织多呈纤维化样改变。（2）各组髓核细胞中水通道蛋白1表达均明显高于同组纤维环细胞中的表达(P≤0.01)。（3）实验组突出型椎间盘标本髓核及纤维环细胞中水通道蛋白1表达明显低于对照组(P＜0.01)，脱出型和游离型椎间盘标本中水通道蛋白1表达明显低于突出型 (P＜0.01)。结论 水通道蛋白1随着椎间盘突出、脱出、游离不同程度退变，表达呈逐渐下降趋势。 |
| 英文摘要: |
| Objective To observe the changes of the expression of aquaporin-1 (AQP-1) in various degenerative human intervertebral discs, and to investigate its relationship with intervertebral disk degeneration. Methods Thirty intervertebral disc samples were collected from patients with prolapse of lumbar intervertebral disc during operation, including protrusion, extrusion and sequestration type and 10 samples for each type. Ten intervertebral disc samples in control group were collected from patients who had lumbar vertebral fractures. The expression of AQP-1 in lumbar intervertebral disc was detected using immunohistochemistry method. Results The result of hematoxylin and eosin staining showed that the intervertebral disc tissue in control group still maintained a dense lamellar structure, while the intervertebral disc tissue in study group presented fibrotic changes. The expression of AQP-1 in nucleus cells in both groups was significantly higher than that in fibrous ring cells (P≤0.01). The expression of AQP-1 in the protrusion specimen, including nucleus cells and fibrous ring cells, in study group was significantly lower than that in control group (P＜0.01). And the expression of AQP-1 in both extrusion type and sequestration type specimen was significantly lower than that in protrusion type specimen (P＜0.01). Conclusion The expression of AQP-1 decreases along with protrusion, extrusion, and sequestration stages of the intervertebral disc degeneration. |
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