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| 尿酸对人骨髓间充质干细胞成骨分化中Cbfα1/Runx2表达的影响 |
| Effect of uric acid on the expression of Cbfα1/Runx2 during the osteogenic differentiation of human bone marrow mesenchymal stem cells |
| 投稿时间：2012-10-17 |
| DOI： |
| 中文关键词: 尿酸 人骨髓间充质干细胞 成骨分化 Cbfα1/Runx2 |
| 英文关键词:Uric acid HBMSCs Osteogenic differentiation Core-binding factor α1 |
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| 中文摘要: |
| 目的 观察不同浓度尿酸对人骨髓间充质干细胞(hBMSCs)成骨分化过程中核心结合因子α1(Cbfα1/Runx2)表达变化的影响.方法 以体外培养的健康成年hBMSCs为研究对象,分为5个组,分别为对照组(完全培养基组)和加入不同浓度尿酸(0 mmol/l、0.2 mmol/l、0.4 mmol/l、0.8mmol/l)的成骨诱导组,通过倒置显微镜观察细胞形态,碱性磷酸酶染色和茜素红染色鉴定细胞.在干预诱导第7天和第14天行RT-PCR检测Cbfα1/Runx2的表达.结果 碱性磷酸酶染色和茜素红染色结果均阳性,表示诱导后细胞为成骨细胞.RT-PCR结果表明,对照组各时间点均无Cbfα1/Runx2表达,尿酸培养组随尿酸浓度增加和时间的延长,Cbfα1/Runx2表达逐渐增强,呈现时间依赖性和浓度依赖性.结论 尿酸可能通过促进Cbfα1/Runx2的表达,从而促进hBMSCs向成骨细胞分化. |
| 英文摘要: |
| Objective To investigate the effect of different concentrations of uric acid (UA) on the expression of core-binding factor α1 (Cbfα1/Runx2) during osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into osteoblasts in vitro. Methods HBMSCs from healthy adults cultured in vitro were
studied and divided into 5 groups, including 1 control group (standard serum-containing medium) and 4 osteogenic induction groups (the medium contained 0, 0.2, 0.4, and 0.8 mmol/L UA, respectively). Cell morphology was observed using a phase-contract microscope. Alkaline phosphatase (ALP) staining and alizarin red staining were performed to identify the cells. The expression of Cbfα1/Runx2 was tested using RT-PCR at the 7th and 14th day after the intervention. Results ALP staining and alizarin red staining were positive, indicating the cells after induction were osteoblasts. The results of RT-PCR showed no expression of Cbfα1/Runx2 in control group at the 7th and 14th day. The expression of Cbfα1/Runx2 increased along with the increase of UA concentrations and time in osteogenic induction groups, presenting a dose-dependent and time-dependent pattern. Conclusion UA can promote the differentiation of hBMSCs into osteoblasts by promoting the expression of Cbfα1/Runx2. |
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