| Objective To investigate the dynamic changes of the expression of Cbfa1 and OCN in osteoporotic fracture healing progress promoted by the transplantation of adipose-derived stem cells (ADSCs), which were cultivated in Gukang-containing serum. Methods Gukang-containing serum was prepared and used for the culturing of ADSCs. And then the ADSCs were transplanted to the fracture site. The dynamic changes of Cbfa1 and OCN were observed using immunohistochemistry. Results The third passage of ADSCs were identified as CD90 positive, CD34 and CD45 low expression. The dynamic changes of Cbfa1 and OCN could be observed using immunohistochemistry. At the first week, hematoma, granulation tissue, Cbfa1 and OCN-positive cells, and matrix positive signal in fibrous callus could be observed in intervention group, while positive cells and matrix positive signal in control group could be observed, but not as strong as in intervention group. And no positive expression was observed in blank group. At the second week, cartilage islands and new trabecular bone were observed in intervention group, and collagen fibers arranged in neat rows. Large number of Cbfa1 and OCN-positive cells and matrix positive signal could be observed, showing a strong positive expression. Trabecular bone and cartilage islands were also observed in control group, but in less extent than that in intervention group. Less strong positive expression of positive cells and matrix positive signal was observed in control group. While rare cartilage islands and trabecular were observed in blank group, chaotic arrangement of collagen fibers, least positive cells, and weakest matrix positive signal in 3 groups were also observed. At the fourth week, the expression of Cbfa1 decreased, while the strong expression of OCN continued in intervention group. In control group, the two indicators showed positive expression. In blank group, positive cells and matrix positive signal showed much stronger than those previously. Conclusion Through the observation of dynamic changes of the Cbfa1 and OCN expression using immunohistochemistry in the fracture sites, we find that the transplantation of ADSCs can promote osteoporotic fracture healing process. The transplantation of ADSCs cultivated in Gukang-containing serum has better effect. |