| Objective To investigate the effect of glucagon like peptide-1 (GLP-1) analogues (Liraglutide) on the proliferation of the human osteoblasts and the mRNA expression of Wnt signaling pathway-related factors, and to explore the relationship between GLP-1 and bone metabolism. Methods The different concentrations of GLP-1 analogues (0 mol/L, 10-7 mol/L, 10-8 mol/L, and 10-9 mol/L) were additioned to human osteoblast culture in vitro. Cell proliferation was determined using cell counting kit-8 (CCK-8) after 24h. The mRNA expression levels of Wnt-3a, LRP-5, and β-catenin were detected using real time polymerase chain reaction (Real-time RT-PCR). Results GLP-1 could promote the proliferation of human osteoblasts (P<0.05), while the proliferation rate of osteoblasts declined along with the concentration increase (P<0.05). Low concentration of GLP-1 (10-9mol/L) could up-regulate the mRNA expression levels of Wnt-3a, LRP-5, and β-catenin in human osteoblasts (P<0.05). In contrast, high concentrations of GLP-1 (10-7mol/L and 10-8mol/L) could down-regulate the mRNA expression levels of Wnt-3a, LRP-5, and β-catenin in human osteoblasts (P<0.05). Conclusion GLP-1 can promote the proliferation of human osteoblasts. When at low concentrations, Wnt signaling pathway may be involved in the regulatory process, while at high concentrations, it may inhibit the expression of Wnt signaling pathway-related genes. |