|
| 牛骨胶原蛋白肽促进HOB增殖 |
| Bovine collagen peptides promotes proliferation of human osteoblasts |
| |
| DOI：10.3969/j.issn.1006.7108.2013.07.011 |
| 中文关键词: 胶原蛋白肽 人成骨细胞 增殖 |
| 英文关键词:Bovine collagen peptides HOB Proliferation |
| 基金项目:中国科学院理化技术研究所-东宝生物合作课题(编号:DBSW2013-01),总装后勤部课题资助项目 |
|
| 摘要点击次数: 791 |
| 全文下载次数: 1259 |
| 中文摘要: |
| 目的 研究牛骨胶原蛋白肽( Bovine Collagen peptides, BCP)对人成骨细胞(Human Osteoblast,HOB)增殖的影响。方法 分离培养HOB，茜素红染色鉴定HOB的成骨特性，利用MTT实验检测胶原蛋白肽对HOB的增殖，流式细胞仪测定胶原蛋白肽对HOB细胞周期。结果 茜素红矿化染色结果表明，HOB具有成骨能力。MTT实验表明0. 3 mg/ml胶原蛋白肽能显著促进细胞的增殖，并能增加G2+S期的百分含量。结论 牛骨胶原蛋白肽能够促进HOB增殖，为骨质疏松的预防和治疗提供理论依据。 |
| 英文摘要: |
| Objective To investigate the effect of bovine collagen peptides(BCP)on the proliferation of human osteoblasts(HOB).Methods HOBS were isolated and cultured. The osteogenesis of HOB was identified using Alizarin red staining. The effect of BCP on proliferation of the cells was detected using MTT method. The effect of BCP on cell cycle of HOB was examined using flow cytometry. Results Alizarin red staining showed that HOB had osteogenic ability. The results of MTT showed that 0. 3 mg/ml BCP could significantly promote HOB proliferation. And the results of flow cytometry showed that 0. 3 mg/ml BCP could increase the percentage of G2+S phase. Conclusion Bovine collagen peptide can promote HOB proliferation, which may provide a theoretical basis for the prevention and the treatment of osteoporosis. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |