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| 骨吸收抑制剂对卵巢切除骨折大鼠脂类代谢及骨钙素的影响 |
| Effect of bone resorption inhibitors on blood lipid metabolism and serum osteocalcin in ovariectomized rats with fractures |
| 投稿时间：2012-09-06 |
| DOI： |
| 中文关键词: 骨吸收抑制剂 绝经后骨质疏松 骨钙素 脂类代谢 |
| 英文关键词:Bone resorption inhibitors Postmenopausal osteoporosis Osteocalcin Lipid metabolism |
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| 中文摘要: |
| 目的 研究骨吸收抑制剂对卵巢切除大鼠脂类代谢及骨钙素的影响.方法 雌性SD大鼠共140只,随机分为5组,每组28只.3月龄时选取其中4组大鼠行双侧卵巢切除建立绝经后骨质疏松症模型(OVX组、OVX+ EE2组、OVX+ Rlx组、OVX+ Aln组),另一组行假手术(Sham组).OVX组及Sham组皮下注射生理盐水,余下3组分别注射阿仑膦酸钠(Aln)、雷洛昔芬(Rlx)、雌激素(EE2),每周注射5次.分别在卵巢切除术后4周、10周及20周测定大鼠血脂、骨钙素及相关生化指标.结果 OVX组与Sham组相比体重增加,总胆固醇升高,甘油三脂降低,差异有统计学意义.应用雌激素及雷洛昔芬可有效调节卵巢切除导致的脂代谢紊乱,表现为体重下降及总胆固醇水平降低,差异有统计学意义(P＜0.05).不同时期OVX组骨钙素水平高于Sham组,差异有统计学意义(P＜0.05),其中阿仑膦酸钠组血清骨钙素水平最低.随着时间推移,Sham组及OVX组骨钙素呈现先上升再下降趋势,而OVX+EE2组、OVX+ Rlx组及OVX+ Aln组骨钙素表现为持续降低.结论 骨吸收抑制剂能够降低骨钙素水平,从而降低卵巢切除导致的高骨转换率,防止骨量丢失.此外,雌激素及雷洛昔芬在预防骨丢失的基础上还能够有效调节卵巢切除引起的脂类代谢紊乱. |
| 英文摘要: |
| Objective To investigate the effect of bone resorption inhibitors (Rlxifene, Estrogen, and Alndronate) on blood lipid metabolism and serum osteocalcin in ovariectomized rats. Methods One hundred and forty female Sprague-Dawley rats were randomly divided into 5 groups, and each group had 28 rats. At the 3rd month, rats in 4 groups were selected for the establishment of postmenopausal osteoporosis model by performing bilateral ovariectomy: OVX, OVX+Rlxifene (Rlx), OVX+Estrogen (EE2), and OVX+Alndronate (Aln). Rats in the rest group received sham operation (Sham group). Rats in OVX group and Sham group received percutaneous injection of normal saline. Rats in the other groups received percutaneous injection of Rlx, EE2, or Aln, 5 times per week. Blood lipids, osteocalcin, and other biochemical indexes were detected at the 4th, 10th, and 20th week after the operation. Results Comparing with those in Sham group, body weight and total serum cholesterol (TC) in OVX group were higher (P<0.05), while serum triglyceride (TG) was lower (P<0.05). The application of Rlx and EE2 could effectively regulate the lipid metabolism disorder caused by ovariectomy, showing a decrease of total cholesterol and body weight (P<0.05). Serum osteocalcin in OVX group at different stages was higher than that in Sham group (P<0.05). Among them, serum osteocalcin in OVX+Aln group was the lowest. Along with the time, serum osteocalcin in sham group and OVX group increased at the early stage of fracture healing, and then declined later. While serum osteocalcin in the other three groups showed a tendency of continuous decrease during the whole fracture process. Conclusion Bone resorption inhibitors can lower serum osteocalcin level, in turn lowering the high bone turnover rate caused by ovariectomy, and preventing bone loss. On the basis of preventing bone loss, Ral and EE2 can also effectively regulate the lipid metabolism disorder caused by ovariectomy. |
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