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| 盐酸二甲双胍对人成骨细胞增殖及Collagen I 、 LRP5 mRNA表达的影响 |
| Effect of metformin on the proliferation of human osteoblasts and the mRNA expression of collagen I and LRP5 |
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| DOI： |
| 中文关键词: 二甲双胍 人成骨细胞 增殖 Collagen I ；LRP5 |
| 英文关键词:Metformin Human Osteoblasts Proliferation Collagen I LRP5 |
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| 中文摘要: |
| 目的观察不同浓度二甲双胍对体外培养人成骨细胞增殖、Collagen I及LRP5mRNA表达的影响，探讨二甲双胍对成 骨细胞作用的可能机制。方法不同浓度二甲双胍(0、25、50、100、200 ^mo^L)刺激体外培养人成骨细胞72 h，采用CCK~8比 色法检测细胞增殖情况，用荧光定量RT~PCR法检测Collagen I、LRP5mRNA表达。结果显示二甲双胍可促进其增殖（P < 0.05)，并促进成骨细胞CollagenI及LRP5mRNA表达（P <0.05)，在200 pmoVL时增殖、表达最明显，呈剂量效应关系。结 论二甲双胍在一定浓度范围内可促进人成骨细胞增殖，可能通过Wnt/LRP5信号通路增强Collagen I及LRP5mRNA的表达 来调控。 |
| 英文摘要: |
| Objective To observe the effect of different concentrations of metformin on the proliferation of human osteoblasts and the mRNA expression of collagen I and LRP 5，and to investigate the possible mechanism of the effect of metformin on human osteoblasts . Methods Human osteoblasts cultured in vitro were treated with different concentrations of metformin (0，25，50， 100， and 200 pmol/L) for 72 hours. Then cell proliferation was assessed with CCK -8 colorimetric method. The mRNA expression of collagen I and LRP5 was detected using fluorescent quantitative RT -PCR. Results Metformin could promote the proliferation of osteoblasts (P < 0.05)，and also could promote the mRNA expression of collagen I and LRP 5 (P < 0. 05). The promotion effect was highest at the concentration of 200^ mol/L，showing a dose -dependent manner. Conclusion In a certain range of concentration，metformin can promote the proliferation of human osteoblasts . It may regulate this progress by enhancing the mRNA expression of collagen I and LRP 5 through the Wnt/LRP5 signal pathway. |
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