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| 骨康方含药血清对骨髓基质细胞成骨分化过程中 I型胶原基因表达的影响 |
| Effect of GuKang containing serum on the expression of COL I gene during the differentiation of bone marrow stromal cells into osteoblasts |
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| DOI： |
| 中文关键词: 骨康 骨髓基质细胞 分化I型胶原 |
| 英文关键词:GuKang Bone marrow stromal cells Differentiation COL I |
| 基金项目: |
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| 中文摘要: |
| 目的观察骨康方含药血清对体外培养骨髓源性骨髓基质细胞（Bone Marrow Stromal Cells，BMSCs )中I型胶原的影 响。方法采用梯度离心法获取大鼠骨髓源性MSCs，随机将实验用大白兔分为骨康方高、中、低3个剂量组，对实验用大白兔 给药干预7 d后，通过血清药理学的方法使用各组大白兔血清联合诱导剂培养传6代BMS&2周后，RT-PCR方法检测I型胶 原mRNA表达。结果高、中浓度骨康方含药血清加诱导剂作用于体外培养的BMSCs2周后，能使BMSCs中I型胶原mRNA 的表达升高。结论具有补肾填精壮骨作用的骨康方可以升高大鼠体外BMSCs向成骨细胞分化过程中I型胶原mRNA的表 达，这可能是防治骨质疏松症的机理之一。 |
| 英文摘要: |
| Objective To observe the effect of GuKang containing serum on the expression of COL I gene during the differentiation of bone marrow stromal cells ( BMSCs) into osteoblasts in vitro. Methods Rat BMSCs were collected using gradient centrifugation . The experimental rabbits were randomly divided into high concentration ，middle concentration，and low concentration of GuKang groups. After a 7 -day intervention， GuKang containing serum was collected from rabbits . BMSCs were cultured with the conditioned serum combined with the inductor for 2 weeks，with 6 passagings. The expression of collagen I mRNA was detected using RT -PCR method. Results After cultured with high concentration and middle concentration of GuKang containing serum combined with the inductor for 2 weeks，the expression of COL I mRNA in BMSCs increased significantly . Conclusion GuKang，which has the function of kidney - tonifying，essence-replenishing，and bone -strengthening，can increase the expression of COL I mRNA during the differentiation of rat BMSCs into osteoblasts in vitro. This may be one of the mechanisms to prevent and to treat osteoporosis . |
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