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| 联合甲状旁腺激素和普萘洛尔对人成骨细胞增殖及OPG和RANKL mRNA表达的影响 |
| Effect of parathyroid combined with propranolol on the proliferation of human osteoblasts and the mRNA expression of OPG and RANKL |
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| DOI：10.3969/j.issn.1006.7108.2013.11.004 |
| 中文关键词: 甲状旁腺素 普萘洛尔 人成骨细胞 骨保护素 核因子kB受体活化因子配体 |
| 英文关键词:Parathyroid hormone Propranolol Human osteoblast Osteoprotegrin(OPG) Receptor activator of NF-KB ligand (RANKL ) |
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| 中文摘要: |
| 目的 联合甲状旁腺激素(rhPTH 1-34)和普萘洛尔(PRO)处理体外培养的人松质骨源性成骨细胞(HOB)，观察其对人成骨细胞增殖及骨保护素(OPO)和核因子KB受体活化因子配体(RANKL )基因表达的影响，探讨其对骨代谢影响的可能机制。方法 (1)以成人骼骨和股骨颈部松质骨为原料，分离培养原代人成骨细胞并对其鉴定。(2)以人成骨细胞为体外实验模型，固定浓度rhPTH1- 34 (50 ng/ml)和不同浓度PRO(0. 1μM,1μM,10μM)分别及联合刺激体外培养的人成骨细胞72 h后，采用CCK-8法检测细胞增殖能力，用实时荧光定量PCR法检测成骨细胞OPG和RANKL基因的表达。结果 rhPTH1-34和PRO单独给药均可促进成骨细胞增殖(P <0.05)，在PRO 10μM时成骨细胞OPG基因的表达量增加(P<0.05)，且大于RANKL基因的表达量;相反，联合rhPTH 1-34和PRO给药抑制成骨细胞增殖(P<0.05)，并抑制成骨细胞OPG和RANKL基因的表达(P<0.05)，且随着PRO浓度的增加，OPG和RANKL基因表达均呈下降趋势。结论 rhPTH1- 34和PRO单独给药均可促进成骨细胞增殖，而两者联合给药后却抑制了成骨细胞的增殖，可能是通过调控OPG和RANKL基因的表达来实现的。 |
| 英文摘要: |
| Objective To observe the effect of parathyroid(rhPTH1-34)combined with propranolol(PRO)on the proliferation of human osteoblasts(HOB) in vitro and the mRNA expression of OPG and RANKL，and to investigate the possible mechanism in bone metabolism. Methods The cancellous bone of the ilium and the femoral neck from adult patients were collected .Then，primary human osteoblasts were isolated，cultured in vitro，and identified. Human osteoblasts were treated with a 50 ng/ml of rhPTH1-34 combined with different concentrations (0. 1μM，1μM，and 10μM ) of propranolol. After 72 hours，cell proliferation was detected using CC-8，and the mRNA expression of OPG and RANKL was detected using real time quantitative PCR .Results The monotherapy of rhPTH1-34 or PRO could promote the proliferation of osteoblasts(P<0.05)，and the expression of OPG mRNA increased with 10μM PRO(P<0.05)，which was higher than that of RANKL mRNA .In the contrary，combined treatment of rhPTH1-34 and PRO inhibited cell proliferation(P < 0. 05 ) and the mRNA expression of OPG and RANKL(P < 0. 05).Along with the increase of PRO concentration，the mRNA expression of both OPG and RANKL showed a tendency of decrease .Conclusion The monotherapy of rhPTH1-34 or PRO can promote the proliferation of osteoblasts，while the combined treatment can inhibit the proliferation of osteoblasts，which may act through regulating the mRNA expression of OPG and RANKL mRNA. |
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