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| 骨碎补提取物对骨髓间充质干细胞向成骨细胞分化Cbfα-1表达的影响及意义 |
| Effect of rhizoma drynariae extract on Cbf alpha 1 expression during the differentiation of osteoblasts by bone marrow mesenchymal stem cells and its significance |
| 投稿时间：2012-11-13 |
| DOI： |
| 中文关键词: 骨碎补 骨髓间充质干细胞 Cbfα-1 成骨能力 |
| 英文关键词:Rhizoma drynariae Bone marrow mesenchymal stem cells Cbf alpha 1 Osteogenesis ability |
| 基金项目:山东省中医管理局（2005-172） |
| 作者 | 单位 | E-mail | | 刘国岩 | 山东中医药大学附属医院骨科,济南,250011 | lgy0531@163.com | | 徐展望 | 山东中医药大学附属医院骨科,济南,250011 | | | 徐琬梨 | 山东中医药大学基础医学院,济南,250355 | | | 张建新 | 山东中医药大学附属医院骨科,济南,250011 | | | 刘彬 | 山东中医药大学,济南,250355 | |
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| 中文摘要: |
| 目的：体外实验研究中药骨碎补对大鼠骨髓间充质干细胞增殖分化及对Cbfα-1表达的影响。方法采用中药干预培养细胞的方法，利用骨碎补单味药物与培养基联合培养大鼠骨髓间充质干细胞（ BMSCs ），通过Ⅰ型胶原免疫组化染色检测其促BMSCs成骨分化能力，通过RT-PCR方法进行半定量分析Cbfα-1的表达，探讨中药骨碎补促成骨能力的机制。结果骨碎补药物各组Ⅰ型胶原免疫组化染色检测BMSCs成骨分化能力及RT-PCR检测Cbfα-1的表达均明显优于空白组及条件培养组（P＜0.01），0.002 g/ml药物组优于0.02 g/ml及0.0002 g/ml药物组（P＜0.05）。结论中药骨碎补可促进BMSCs增殖及成骨分化，使Cbfα1的表达加强。 |
| 英文摘要: |
| Objective To investigate the effect of traditional Chinese medicine ( TCM) , rhizoma drynariae, on the proliferation and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) and the expression of Cbf alpha1 (Cbfα-1) in vitro. Methods The method of culturing cells with the intervention of TCM was used.Rat BMSCs were cultured with the combination of single drug rhizoma drynariae and the medium.Collagen type I immunohistochemical staining was performed to detect its ability to promote the osteogenesis of BMSCs.The expression of Cbfα-1 was semi-quantitatively analyzed using RT-PCR, in order to investigate the mechanism of rhizoma drynariae promoting osteogenesis.Results The ability to promote the osteogenesis of BMSCs detected using collagen type I immunohistochemical staining and the expression of Cbfα-1 detected using RT-PCR in all rhizoma drynariae drug groups were significantly better than that in blank control group and conditioned culture group ( P<0.01 ) . The effect in the group with 0.002 g/ml of the drug was better than that in the groups with 0.02 g/ml and 0.0002 g/ml of the drug (P<0.05).Conclusion Rhizoma drynariae can promote the proliferation and osteogenesis differentiation of BMSCs and enhance the expression of Cbfα-1. |
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