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| 骨炎康颗粒对膝骨性关节炎大鼠血清 II 型胶原、TGF-β1、IL-lβ作用的实验研究 |
| Experimental study of the effect of Guyankang granules on the expression of serum type II collagen, TGF-β1, and IL-1βin rats with knee osteoarthritis |
| 投稿时间：2013-01-21 |
| DOI： |
| 中文关键词: 骨性关节炎 骨炎康颗粒 血清II型胶原 TGF-β1 IL-lβ |
| 英文关键词:Osteoarthritis Guyankang granules |
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| 中文摘要: |
| 目的：通过对膝骨性关节炎（ KOA）模型大鼠的实验，观察复方中药骨炎康颗粒对膝骨性关节炎的作用机制。方法选用SD大鼠，随机分为正常对照组（A组）、模型组（B组）、骨炎康颗粒治疗组（C组）、西药对照组（D组）。通过左膝关节腔内注入木瓜蛋白酶，造成骨性关节炎病变模型。木瓜蛋白酶注射结束后2周，开始对C、D组分别予以药物骨炎康颗粒、布洛芬灌胃，8周后处死全部大鼠，取A组双侧膝关节及B、C、D组造模侧膝关节制作病理标本，分别行免疫组化（ TGF-β1、IL-lβ）染色，观察血清II型胶原、关节软骨及白介素l（1L-lβ）、转移生长因子（TGF-β1）的改变。结果（1）血清II型胶原结果：骨炎康颗粒能提高血清II型胶原含量；（2）TGF-β1、IL-lβ改变结果：各治疗组均能提高TGF-β1含量，并降低1L-lβ，其中以骨痹颗粒组效果最佳，布洛芬相对较弱。结论骨炎康颗粒可增进软骨基质的合成，抑制软骨基质的分解，促进受损关节软骨的修复。骨炎康颗粒的疗效机理可能在于通过对机体的细胞因子调节，提高血清II型胶原、关节软骨的TGF-β1含量，降低IL-lβ含量，从而直接或间接促进了损伤关节软骨的修复。 |
| 英文摘要: |
| Objective To investigate the mechanism of Guyankang granules, a compound traditional Chinese medicine, on knee osteoarthritis (KOA) rat model.Methods SD rats were selected and randomly divided into normal control group (Group A), model group ( Group B ) , Guyankang granules treated group ( Group C ) , and western medicine group ( Group D ) .The rat osteoarthritis model was established using the injection of papain into the left knee joint cavity.Two weeks after the injection of papain, rats in Group C and Group D were given a gavage of Guyankang granules and ibuprofen, respectively.Eight weeks later, all the rats were executed.The bilateral knees in Group A and model lateral knee in Group B, Group C, and Group D were collected and made into pathological specimen, respectively.The immunohistochemical staining of transforming growth factorβ1 (TGF-β1) and interleukin 1β( IL-lβ) was performed.The changes of serum type II collagen, the articular cartilage, IL-lβ, and TGF-β1 were observed.Results Guyankang granules could improve the content of serum type II collagen.The results of the changes of TGF-β1and IL-lβrevealed that the content of TGF-β1 in each treatment group increased significantly, while the content of IL-lβreduced. The effect in Guyankang Group was the best, while the effect in ibuprofen group was relatively weak.Conclusion Guyankang granules can promote the synthesis of the cartilage matrix, inhibit the decomposition of the cartilage matrix, and promote the repair of the damaged articular cartilage.The possible mechanism of Guyankang granules may be the regulation of cytokines, thus improving the content of serum type II collagen and the content of TGF-β1 in the articular cartilage, reducing the content of IL-lβ, then promoting the repair of the injured articular cartilage directly or indirectly. |
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