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| 利塞膦酸钠联合脉冲电磁场治疗去势大鼠骨质疏松的 实验研究 |
| Experimental study of risedronate combined with pulsed electromagnetic fields for the treatment of osteoporosis in ovariectomized rats |
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| DOI： |
| 中文关键词: 去势 利塞膦酸钠 骨质疏松 脉冲电磁场 |
| 英文关键词: |
| 基金项目:广西科学研究与技术开发计划课题基金资助（桂科攻10124001—64);广西科学自然基金项目（0991287) |
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| 中文摘要: |
| 目的观察利塞膦酸钠联合脉冲电磁场对去势大鼠骨质疏松的疗效。方法将32只雌性SD大鼠(250 ±20) g随机分 成4组:正常生长组(Sham)、去卵巢模型组（OVX)、利塞膦酸钠治疗组（RIS)和利塞膦酸钠联合骨质疏松治疗仪治疗组（RIS + PEMFs)，每组8只。后三组大鼠行双侧卵巢切除术。去势后12 w RIS组给予0. 1 mg- kg、d 1利塞膦酸钠灌胃治疗，RIS + PEMFs组给予0. 1 mg- kg、d 1利塞膦酸钠灌胃和1h> d 1脉冲电磁场治疗，Sham、OVX组每天给予等量生理盐水灌胃。去势 后12 w、灌胃治疗4 w、8 w、12 w后四组大鼠均用双能X线吸收法骨密度仪测量全身骨密度（丽D)，治疗12 w后进行血生化 检测、骨CT值测量和骨组织形态学观察。结果去势后12w后三组BMD与Sham组比较均显著降低(P <0. 01)，大鼠骨质疏 松模型确立;灌胃治疗12 w后，RIS、RIS + PEMFs两组较OVX组BMD(P <0. 01)、骨CT值(尸<0. 01)均较高，且RIS + PEMFs 组 BMD、骨 CT 值均比 RIS 组高(P < 0. 01); RIS 组血清 Ca、ALP 浓度均较 RIS + PEMFs、Sham 组高(P < 0. 05)，且 RIS + PEMFs 组血清ALP浓度较Sham组相比差异无统计学意义(P > 0. 05);股骨颈病理形态学观察中RIS + PEMFs组的骨小梁形态较完 整，较接近Sham组水平。结论去势后12 w可建立大鼠骨质疏松模型;利塞膦酸钠对去势所致骨质疏松大鼠有治疗作用，其 与脉冲电磁场联合的治疗效果更为显著。 |
| 英文摘要: |
| Objective To observe the clinical efficacy of risedronate combined with pulsed electromagnetic fields (PEMFs) for the treatment of osteoporosis in ovariectomized rats. Methods Thirty-two female SD rats were randomly divided into 4 groups ： sham group (Sham)，OVX group (OVX)，risedronate group (RIS)，and risedronate combined with PEMFs group (RIS + PEMFs). Each group had 8 rats. Rats in the last 3 groups received the operation of bilateral ovariectomy. At the 12th week after the operation， rats in RIS group were given a gavage of 0. 1mg kg 1 d 1 risedronate，and rats in RIS + PEMFs group were received a gavage of 0. 1mg kg 1 d 1 risedronate and 1h d 1 pulsed electromagnetic field therapy. Rats in Sham group and OVX group were given a gavage of same volume of normal saline. Bone mineral density (BMD) of the total body was detected using dual energy X^ay absorptiometry at the 12thweek after ovariectomy and the 4th week，the 8th week，and the 12thweek after the gavage. After 12 - week treatment，the bone biochemical detection， bone CT detection， and the histomorphological observation of bone tissues were performed. Results At the 12th week after ovariectomy，BMD in the last 3 group was significantly lower than that in Sham group (P <0. 01)，indicating the establishment of rat osteoporosis model. After 12-week gavage，BMD in RIS group and RIS + PEMFs group and the values of bone CT were higher than those in OVX group (P <0.01)，and the values in RIS + PEMFs group were also |
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