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不同强度静磁场间歇曝磁对骨髓间充质干细胞增殖及 细胞周期的影响 |
Effect of different intensity static magnetic field on cell proliferation and cell cycle by bone marrow mesenchymal stem cells |
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DOI: |
中文关键词: 静磁场 骨髓间充质干细胞 增殖 细胞周期 |
英文关键词:Static magnetic fields Bone marrow mesenchymal stem cells Proliferation Cell cycle |
基金项目:深圳市科技研发资金基础研究计划项目 (JC201005280537 A) |
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中文摘要: |
目的观察不同强度静磁场对骨髓间充质干细胞(MSCs)增殖及细胞周期的影响。方法体外分离和培养大鼠MSCs, 取第三代MSCs,分为对照组与0. 008T磁场组、0. 38T磁场组、0. 48T、0. 68T磁场组。磁场组以24h为曝磁时间间隔,隔天 CCK-8法检测各组细胞增殖活力,流式细胞仪测定各组细胞增殖动力及细胞周期变化。结果0. 008T、0. 38T、0. 48T,0. 68T 静磁场间歇曝磁均能明显促进大鼠MSCs增殖活力(p <0.05),提高细胞的增殖速度和增殖指数。MSCs经0. 008T、0. 38T、 0.68T静磁场24 h间歇曝磁4 d后,各磁场组细胞(S + G2/M)期细胞比例均有不同程度显著提高(p <0.05,p <0. 01)。结论 一定强度的静磁场间歇曝磁能够有效促进细胞增殖,降低G0/G1期的细胞比例。 |
英文摘要: |
Objective To observe the effect of different intensity static magnetic fields (SMFs) on the proliferation and cell cycle by rat bone marrow mesenchymal stem cells (BMSCs). Methods The rat BMSCs were isolated and cultured in vitro. The 3rd passage of BMSCs were selected and divided into 5 groups : control group,0. 008T SMFs group,0. 38T SMFs group,0. 48T SMFs group,and 0. 68T SMFs group. The interval was 24 hours. The proliferation activity of BMSCs was detected using CCK-8 method every other day. The proliferation kinetics and cell cycle of BMSCs were detected using flow cytometry. Results Compared with that in control group,the proliferation of BMSCs in 0. 008T SMFs group,0. 38T SMFs group,0. 48T SMFs group,and 0. 68T SMFs group increased significantly (P <0. 05),and the proliferation frequency and proliferation index also improved. The percentage of cells at the S + G2/M phase increased significantly to some extent after 24h interval exposure to 0. 008T,0. 38T,and 0. 68 T SMFs for 4 days (P <0. 05,P <0. 01). Conclusion Interval exposure to certain intensity SMFs can effectively promote the proliferation of rat BMSCs and decrease the percentage of cells at the G0/G1 phase. |
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