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| 淫羊藿调节BMP-7预防糖皮质激素致大鼠骨损害的 研究 |
| Study of the effect of epimedium on the prevention of glucocorticoid-induced bone lesions by regulating bone morphogenetic protein-7 in rats |
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| DOI： |
| 中文关键词: 淫羊藿 糖皮质激素 骨质疏松 骨形态发生蛋白A |
| 英文关键词:Epimedium Glucocorticoid Osteoporosis Bone morphogenetic protein-7 |
| 基金项目:湛江市科技计划项目（2010 C3102002)；广东医学院 面上项目（XK1002) |
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| 中文摘要: |
| 目的观察醋酸泼尼松和淫羊藿对大鼠胫骨近端上段骨组织形态计量学参数、股骨组织形态学和股骨骨形态发生蛋 白-7(BMP-7)表达的影响，探讨淫羊藿预防糖皮质激素致骨损害的fflMP-7机制。方法3月龄SD大鼠24只，随机分为正常 对照组、糖皮质激素模型组和淫羊藿防治组，灌胃给药90 d。测量胫骨近端上段骨组织形态计量学参数，苏木素4尹红染色法 观察股骨组织形态学，免疫组化法检测股骨BMP9的表达。结果醋酸泼尼松可导致大鼠胫骨近端上段标记周长百分数(％ L. Pm)和骨形成率(BFR/TV、BFR/BV和BFR/BS)减少(P <0. 05);股骨骨小梁钙化减少，成软骨化，纤维组织增生，骨小梁变 窄、数量减少;股骨BMP-7表达减少(P < 0. 05)。与泼尼松组比较，淫羊藿组胫骨近端上段骨小梁面积百分数（% Tb. Ar)和骨 形成率(BFR/BS)增加(P <0.05)，股骨无明显病理损害，股骨丽PJ表达增多(P <0.05)。结论淫羊藿能通过上调骨组织 BMP^7的表达，预防糖皮质激素所致的骨损害。 |
| 英文摘要: |
| Objective To observe the effect of prednisone acetate and epimedium (EP) on bone histomorphometric parameters in proximal tibia metaphyseal (PTM)，the histomorphology of the femur，and the expression of bone morphogenetic protein-- (BMP- 7) in the femur，and to explore the mechanism of EP in preventing glucocorticoid (GC) "induced bone lesions by regulating BMP- 7. Methods Twenty-four 3-month SD rats were randomly divided into three groups ： the normal control group，the GC model group，and the EP prevention group. All the rats were given an intragastric administration of drugs for 90 days. Bone histomorphometric parameters were measured in PTM. Hematoxylin-Eosin staining was performed to observe the histomorphology of the femur. Immunohistochemical method was used to detect the expression of BMP-- in the femur. Results The bone mark perimeter rate (% L. Pm) and the bone formation rate ( BFR/TV，BFR/BV，and BFR/BS) in PTM in the GC model group decreased (P <0.05). The calcification of the bone trabecula decreased， showing chondrocation and fibroplasias. The bone trabecula thinned and decreased. The expression of BMP-7 decreased in the femur (P <0.05). Compared to that in GC model group，the bone trabecular area percentage (% Tb. Ar) and the bone formation rate ( BFR/BS) increased in PTM in the EP prevention group (P < 0. 05). No pathematological lesions were observed. The expression of BMP-- in the femur increased (P < 0.05). Conclusion EP can prevent GC-induced bone lesions by up-iegulating the expression of BMP-- in the bone tissue. |
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