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| 2型糖尿病对小鼠骨代谢的影响 |
| Effect of type 2 diabetes mellitus on bone metabolism ： an in vivo study |
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| DOI： |
| 中文关键词: 2型糖尿病；骨代谢 骨量减少 |
| 英文关键词:Type 2 diabetes mellitus Bone metabolism Osteopenia |
| 基金项目:国家自然科学基金资助项目（81070691) |
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| 中文摘要: |
| 目的观察2型糖尿病小鼠的骨代谢及骨微结构的特点。方法采用雄性KK/UpMy/J小鼠（自发性2型糖尿病模型 小鼠)10只作为实验组，同时选用10只雄性C57BL/6小鼠作为对照组。两组小鼠均给予常规饲料喂养，确认KK/UpMy/J小 鼠发病，继续饲养12周后处死所有小鼠，测定血清骨碱性磷酸酶（BALP)及抗酒石酸酸性磷酸酶(TRAP)活性，并运用Micro- CT分析小鼠胫骨微结构定量参数。结果与对照组相比，2型糖尿病小鼠血清BALP活性明显下降（分别为对照组：0. 029 士 0.003 pU/min，T2DM 组：0. 014 ± 0. 003 pU/min，尸 <0.05)，血清 TRAP 活性明显升高(分别为对照组：0. 513 ± 0. 034 U/L， T2DM 组：0. 701 ±0.054 U/L，尸 <0. 05)，胫骨平台处骨密度明显下降（对照组：810. 000 ±21. 000 mg/cm3，T2DM 组：709. 000 士 18. 000 mgjm3，P <0.05)。结论 2型糖尿病小鼠的骨吸收加快而骨形成不足，导致其骨量下降及骨折风险增大。 |
| 英文摘要: |
| Objective To observe the effect of type 2 diabetes mellitus (T2DM) on bone metabolism and bone microstructure in mice. Methods Ten male KK/Upj~Ay/J mice，a mouse model of T2DM，were selected as experimental group，and 10 male C57BL/6 mice were selected as control group. The mice in both groups were fed with routine fodder. After the confirmation of T2DM in KK/Upj-Ay/J mice，all mice were fed for another 12 weeks and then executed. The activity of serum bone alkaline phosphatase (BALP) and tartrate-resistant acid phosphatase (TRAP) were detected. The parameters of the tibia were analyzed using micro-CT. Results Compared with those in control group， the activity of serum BALP in T2DM mice decreased significantly (control group ： 0. 029 士 0. 003 |jlU/min； T2DM group ： 0. 014 士 0. 003 |jlU/min，P <0. 05)，while the activity of serum TRAP increased significantly (control group ： 0. 513 ± 0. 034 U/L ； T2DM group ： 0. 701 ±0. 054 U/L，P < 0. 05). The bone mineral density of the tibial plateau decreased significantly in T2DM group compared with that in control group (control group ： 810. 000 士 21. 000 mg/cm3 ； T2DM group ： 709. 000 士18. 000 mg/cm3，P < 0. 05). Conclusion The bone resorption in mice with T2DM accelerates while the bone formation is deficient，resulting in osteopenia and increased risk of bone fracture. |
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