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| 补肾活血方调控 microRNA-210促进 BMSCs 成骨分化的研究 |
| The kidney-tonifying and blood circulation-promoting recipes promote the osteogenic differentiation of bone mesenehymal stem cells by regulating microRNA-210 |
| 投稿时间：2013-05-14 |
| DOI： |
| 中文关键词: 骨髓间充质干细胞 miR-210 补肾活血 |
| 英文关键词:Bone mesenehymal stem cells miR-210 Kidney-tonifying and blood circulation-promoting recipes |
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| 中文摘要: |
| 目的：研究补肾活血方促进骨髓间充质干细胞（Bone Mesenehymal Stem Cells ，BMSCs）成骨分化的分子机制。方法贴壁培养法分离纯化大鼠BMSCs，将培养成功的BMSCs分为空白组、成骨诱导组和中药组，培养7d、14d行矿化结节茜素红染色（Alizarin Red S，ARS），倒置显微镜观察矿化骨结节形成情况，采用real-time PCR检测miR-210的表达变化。结果与空白组比较，成骨诱导组和中药组BMSCs钙化结节数量明显增多，差异有非常显著的统计学意义（ P＜0.01）。在成骨诱导剂、补肾活血方水提液的作用下，与空白组比较，miR-210表达明显降低，且中药组较成骨诱导组更明显降低miR-210表达。结论补肾活血方通过降低miR-210表达促进BMSCs成骨分化。 |
| 英文摘要: |
| Objective To investigate the mechanism of osteogenic differentiation of bone mesenehymal stem cells ( BMSCs) promoted by the kidney-tonifying and blood circulation-promoting recipes.Methods BMSCs of SD rats were isolated with adherent separation method and primarily cultured in vitro.Then BMSCs were divided into blank group, osteo-induced group, and Chinese medicine group.At the 7 th day and the 14 th day, the mineralized nodules were stained using Alizarin red statining ( ARS) . The formation of mineralized nodules was observed using a converted microscope.The expression of miR-210 was detected using real-time PCR.Results Compared with that in blank group, the number of mineralized nodules in osteo-induced group and Chinese medicine group increased significantly ( P <0.01 ) .After the intervention of osteogenic inducer and extraction of the kidney-tonifying and blood circulation-promoting recipes, the expression of miR-210 in both groups decreased significantly compared with that in blank group.And the expression in Chinese medicine group was much lower than that in osteo-induced group. Conclusion The kidney-tonifying and blood circulation-promoting recipes can promote the osteogenic differentiation of BMSCs by down-regulating the expression of miRNA-210. |
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