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| 膝关节骨性关节炎患者尿液中C2C水平检测 |
| Detection of urine C2C level in patients with knee osteoarthritis |
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| DOI： |
| 中文关键词: 骨性关节炎 C2C n型胶原 |
| 英文关键词:Osteoarthritis C2C Type II collagen |
| 基金项目:桂林市科学研究与技术开发计划项目(20130120-5) |
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| 中文摘要: |
| 目的观察并探讨尿液中n型胶原羧基末端3/4片段(C2C)水平与膝关节骨性关节炎(KOA)患者病变程度的关系。 方法收集90例膝关节骨性关节炎患者及20例非膝关节骨性关节炎患者清晨尿液标本5 mL及膝关节正侧位影像学资料， 尿液标本置-80 °〔冰箱保存，影像学资料根据Kellgreniawrence放射学分级标准对膝关节骨性关节炎患者的影像学表现进 行分级，实验分为五组，对照组，I级组，II级组，III级组及IV级组。各组间年龄及性别构成比较没有显著性差异，无统计学意 义。尿液标本收齐后采用酶联免疫吸附试验（ELISA)检测尿液中C2C水平。结果与对照组（261. 235 ± 39. 944 pg/mL)相 比，膝关节骨性关节炎组C2C水平(218. 341 ±22. 270 pg/mL)明显升高，差异有统计学意义（P <0. 01)。各组C2C水平比较： IV 组（311. 872 ±18. 759 pg/mL) >III 组（273. 753 ± 18. 595 pg/mL) >II 组（256. 251 ±23. 379 pg/mL) >I 组（219. 009 ± 13. 431 pg/mL)或正常组(218. 341 ±22. 270 pg/mL) (P = 0. 000)。I级膝关节骨性关节炎组患者尿液C2C与对照组水平相差无统计 学意义(P > 0. 05)。结论骨性关节炎患者C2C水平较正常对照组高，C2C升高水平与膝关节骨性关节炎病变程度平行，且 二者呈高度正相关。 |
| 英文摘要: |
| Objective To observe the relationship between the urine C2C level and the severity of knee osteoarthritis (KOA). Methods Ninety patients with KOA were selected as KOA group，and 20 healthy volunteers were selected as control group . Five ml morning urine of each subject was collected and stored at - 80 〇C. The anterior-posterior and lateral position of X -i'ay detection of knee joint was performed. The X~ray images were classified according to the Kellgren -Lawrence radiographic grading criteria. All the subjects were divided into 5 groups ： control group，group I，group II，group III，and group IV，with no significant difference of age and sex. The urine C2C level was detected using enzyme -linked immunosorbent assay (ELISA). Results The urine C2C level in KOA group (218. 341 士 22. 270 pg/mL) was significantly higher than that in control group (261. 235 士 39. 944 pg/mL) (P < 0.01). The urine C2C level in group IV，group III，group II，group I，and control group was 311. 872 士 18. 759 pg/mL，273. 753 ±18.595 pg/mL，256.251 ±23.379 pg/mL，219.009 ± 13.431 pg/mL，and 218.341 ±22.270 pg/mL，respectively，and the difference was significant (P = 0. 000). No significant difference of the urine C2C level between group I and control group was observed (P >0.05). Conclusion The urine C2C level in patients with KOA was higher than that in normal subjects . And the elevation of urine C2C level is parallel to the severity of KOA，showing a high -positive correlation. |
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