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| 珍珠粉对MC3T3-E1细胞增殖、分化、矿化及骨相关基因表达的影响 |
| Effect of pearl powder on the proliferation, differentiation, and mineralization of MC3T3-E1 cells and the expression of bone related genes in the cells |
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| DOI：10.3969/j.issn.1006-7108.2014.06.018 |
| 中文关键词: 珍珠粉 MC3T3-E1细胞 成骨活性 |
| 英文关键词:Pearl powder MC3T3-E1 cell Osteogenic activity |
| 基金项目:2013/12/6 |
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| 摘要点击次数: 1088 |
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| 中文摘要: |
| 目的 研究珍珠粉对MC3T3-E1细胞成骨增殖、分化、间质矿化及凋亡的影响，并初步探讨其作用机制。方法 在体外培养的MC3T3-E1细胞中分别加入珍珠粉和低钙珍珠粉，采用MTT法及碱性磷酸酶(Alkaline phosphatase，ALP)试剂盒检测其对MC3T3-E1细胞增殖及分化的影响；Von Kossa染色法观察矿化结节的形成；RT-PCR检测细胞中转录因子（Runt-related transcription factor II，Runx2）、骨钙素（Osteocalcin，OC）、骨保护因子（Osteoprotegerin, OPG）及核因子-κB受体活化因子配体（Receptor activator of NK-κB ligand, RANKL）mRNA的表达；流式细胞术检测血清饥饿诱导的细胞凋亡。结果 珍珠粉及低钙珍珠粉在一定浓度范围内，能促进MC3T3-E1细胞成熟期的ALP合成分泌和矿化结节的形成，并对Runx2及OC基因表达具有明显促进作用，可降低RANKL/OPG的比率，明显改善血清饥饿诱导的成骨细胞凋亡。结论 珍珠粉及低钙珍珠粉对MC3T3-E1细胞分化成熟过程有显著的促进作用，前者作用显著强于后者，说明珍珠粉中钙成分对MC3T3-E1细胞分化成熟起到主要作用。 |
| 英文摘要: |
| Objective To investigate the effect of pearl powder on the osteogenic proliferation, differentiation, interstitial mineralization, and apoptosis in MC3T3-E1 cells, and to explore the potential mechanisms. Methods MC3T3-E1 cells were cultured with pearl powder or low-calcium pearl powder in vitro. The osteogenic proliferation was determined using MTT method. And the differentiation was determined using alkaline phosphatase (ALP) kit. The formation of mineralized nodules was observed using von Kossa staining. The mRNA expression of Runt-related transcription factor II (Runx2), osteocalcin (OC), osteoprotegerin (OPG), and receptor activator of NK-κB ligand (RANKL) was detected using RT-PCR. The cell apoptosis induced by serum deprivation was detected using flow cytometry. Results In a certain range of concentration, pearl powder and low-calcium pearl powder could significantly increase the secretion of ALP and the formation of mineralized nodules, and markedly improve the mRNA expression of Runx2 and OC in MC3T3-E1cells. Furthermore, pearl powder and low calcium pearl powder could significantly decrease the RANKL/OPG ratio, and ameliorate the apoptosis of the osteoblasts induced by serum deprivation. Conclusion Both pearl powder and low calcium pearl powder can promote the differentiation and maturation in MC3T3-E1cells. And the effect of pearl powder is much stronger, suggesting a critical role of calcium contained in pearl powder in regulating the osteogenic proliferation and differentiation in MC3T3-E1cells. |
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