|
| 补肾益髓中药复方对去卵巢骨质疏松症大鼠骨组织Runx2 mRNA及蛋白表达的影响 |
| Effect of the traditional Chinese herb compound, the reinforcing the kidney to replenish the marrow prescription, on mRNA and protein expression of Runx2 in the bone tissue of ovariectomized osteoporosis rats |
| 投稿时间：2013-12-06 |
| DOI： |
| 中文关键词: 绝经后骨质疏松症 补肾益髓中药复方 Runx2 |
| 英文关键词:Postmenopausal osteoporosis (PMOP) Reinforcing the kidney to replenish the marrow prescription Runx2 |
| 基金项目:辽宁省教育厅科学研究一般项目 (L2011247) |
|
| 摘要点击次数: 982 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：观察去卵巢骨质疏松症大鼠骨组织 Runx2 mRNA 及蛋白表达，探讨绝经后骨质疏松症（ Postmenopausal Osteoporosis，PMOP）的发病机制以及补肾益髓中药复方的疗效机理。方法去卵巢复制骨质疏松症大鼠模型，实验设正常组、模型组、假手术组、补肾益髓中药复方组、钙尔奇D组、骨疏康组。术后7d开始灌胃给药12周。应用XR－36型双能X线骨密度仪测定股骨骨密度，应用OLYMPUS BX51显微镜观察股骨头石蜡切片HE染色显微形态结构，实时定量RT－PCR及Western Blot检测骨组织Runx2 mRNA及蛋白表达。结果（1）与正常组、假手术组比较，模型组股骨骨密度明显降低（ P＜0．001、P＜0．05），骨组织Runx2 mRNA及蛋白表达明显降低（P＜0．001）。（2）与模型组比较，补肾益髓中药复方组、骨疏康组股骨骨密度明显升高（P＜0．05），补肾益髓中药复方组（P＜0．001）、钙尔奇D组（P＜0．05）、骨疏康组（P＜0．01）骨组织Runx2 mRNA及蛋白表达明显上调。（3）与钙尔奇D组、骨疏康组比较，补肾益髓中药复方组骨组织Runx2 mRNA（P＜0．01、P＜0．05）及蛋白（P＜0．001）表达均明显升高。（4）股骨头石蜡切片HE染色显微形态结构：与正常组、假手术组比较，模型组骨小梁形态结构完整性差，有些部位破坏、断裂，余留骨重建空间较多；各给药组骨小梁形态结构均改善，结构较紧密，余留骨重建空间减少，其中以补肾益髓中药复方组改善最明显。结论骨组织Runx2 mRNA及蛋白表达降低可能是PMOP的发病机制之一；补肾益髓中药复方可能通过上调骨组织Runx2 mRNA及蛋白表达有效防治PMOP，其作用优于钙尔奇D、骨疏康。 |
| 英文摘要: |
| Objective To observe the mRNA and protein expression of Runx2 in the bone tissue of ovariectomized osteoporosis rats, to explore the pathogenesis of postmenopausal osteoporosis ( PMOP) , and to investigate the therapeutic effect and mechanism of traditional Chinese herb compound, reinforcing the kidney to replenish the marrow prescription.Methods The osteoporosis rat model was successfully established by ovariectomy.All the rats were divided into normal group, model group, sham-operated group, and reinforcing the kidney to replenish the marrow prescription group, Caltrate D group, and Gushukang group.Seven days after the operation, gavage administration was performed and lasted for 12 weeks.Then the bone mineral density ( BMD) of the femur was detected using dual energy X-ray absorptiometry ( XR-36 ) .The micro-morphology structure of HE stained paraffin sections of the femoral head was observed using microscope ( OLYMPUS BX51 ) .The mRNA expression of Runx2 in the bone tissue was detected using real-time quantitative RT-PCR.The protein expression was detected using Western blotting.Results Compared with that in normal group and sham-operated group, BMD of the femur in model group decreased significantly ( P<0.001, P<0.05).The mRNA and protein expression of Runx2 in the bone tissue decreased significantly (P<0.001).Compared with that in model group, BMD of the femur in reinforcing the kidney to replenish the marrow prescription group and Gushukang group increased significantly (P<0.05).The mRNA and protein expression of Runx2 in bone tissue was up-regulated significantly in reinforcing the kidney to replenish the marrow prescription group (P<0.001), Caltrate D group (P<0.05, and Gushukang group (P<0.01).Compared with that in Caltrate D group and Gushukang group, the mRNA and protein expression of Runx2 in the bone tissue in reinforcing the kidney to replenish the marrow prescription group both increased significantly ( P<0.01, P<0.05;P<0.001) .The observation of the micro-morphology structure of HE stained paraffin sections of the femoral head showed that, compared with that in normal group and sham-operated group, the bone trabeculas in model group had bad morphological integrity with some damaged and fractured parts and more bone remodeling space.The morphology structure of bone trabeculas improved in all administration groups, with more compact structure and less bone remodeling space, and the condition in reinforcing the kidney to replenish the marrow prescription group improved most obviously.Conclusion The down-regulation of the mRNA and protein expression of Runx2 in bone tissues may be one pathogenesis of PMOP.Reinforcing the kidney to replenish the marrow prescription may play an effective role in the prevention and treatment of PMOP by up-regulating the mRNA and protein expression of Runx2 in bone tissues, which is better than Caltrate D and Gushukang. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|