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| 补肾健脾化瘀方对去势大鼠股骨骨髓ERRα和PGC-1α mRNA表达的影响 |
| Effect of kidney-tonifying, spleen-strengthening, and blood stasis removing recipe on the expression ERRα and PGC-1α mRNA in the bone marrow of the femurs in ovariectomized rat model |
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| DOI：10.3969/j.issn.1006-7108.2014.10.001 |
| 中文关键词: 中药 去势大鼠 骨质疏松 雌激素相关受体α 过氧化物酶体增殖物活化受体γ协同刺激因子-1α mRNA |
| 英文关键词:Chinese medicine Ovariectomized rat s Osteoporosis ERRα PGC-1α mRNA |
| 基金项目:国家自然科学基金（81302994，81273778）；广东省科技计划省国际合作项目（2012B050600026）；广东省自然科学基金（S2013040014927） |
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| 中文摘要: |
| 目的 探讨补肾健脾化瘀方对去势大鼠股骨骨髓组织中雌激素相关受体α（ERRα）和过氧化物酶体增殖物活化受体γ协同刺激因子-1α（PGC-1α） mRNA表达的影响。方法 40只7月龄SD雌性大鼠随机分成假手术组、模型组、中药组（补肾健脾化瘀方）、阿伦磷酸钠组。治疗12周后，小动物双能X线检测左侧股骨骨密度，HE染色观察胫骨近端骨形态，QPCR检测骨髓组织中ERRα、PGC-1α mRNA表达水平检测。结果 模型组骨密度低于假手术组（P＜0.05）；中药组与阿伦磷酸钠组骨密度高于模型组（P＜0.05），而假手术组、中药组与阿伦磷酸钠组之间无统计学差异。与假手术组相比，模型组骨髓组织中ERRα mRNA表达水平上升（P＜0.05），PGC-1α mRNA表达水平下降（P＜0.05）。予以药物干预后，与模型组相比，中药组骨髓组织中ERRα mRNA表达水平下降（P＜0.05），PGC-1α mRNA表达水平上升（P＜0.05）；而阿伦磷酸钠组骨髓组织中ERRα mRNA表达水平下降（P＜0.01），PGC-1α mRNA表达水平虽呈现增高的趋势，但无统计学差异（P＞0.05）。结论 补肾健脾化瘀方可能是通过提高骨髓组织中PGC-1α mRNA表达水平，降低ERRα mRNA表达水平，从而提高去势大鼠骨密度。 |
| 英文摘要: |
| Objective To explore the effect of kidney-tonifying, spleen-strengthening, and blood stasis removing recipe on the expression of ERRα and PGC-1α mRNA in the bone marrow of the rat femurs in an ovariectomized rat model. Methods Forty 7-month-old female SD rats were randomly divided into sham-operated group, ovariectomized (OVX) group, herb group (kidney-tonifying, spleen-strengthening, and blood stasis removing recipe), and alendronate (ALEN) group, with 10 rats per group. After a 12-week treatment, the bone mineral density (BMD) of the left femur was measured using small animal special dual energy X-ray absorptiometry. Bone morphology of the proximal tibia was observed using HE staining. The expression of ERRα and PGC-1α mRNA of bone marrow was detected using quantitative PCR. Results Compared with that in sham-operated group, BMD in OVX group was obviously decreased (P<0.05). Compared with that in OVX group, BMD in herb group and in ALEN group was obviously increased (P<0.05). There was no statistically significant among sham-operated, herb and ALEN groups. Compared with sham-operated group, the expression of ERRα mRNA in OVX group was obviously increased (P<0.05) and the expression of PGC-1α mRNA was obviously decreased (P<0.05). After the drug intervention, the expression of ERRα mRNA in herb group was obviously decreased (P<0.05) and the expression of PGC-1α mRNA was obviously increased compared with that in OVX group (P<0.05). The expression of ERRα mRNA in ALEN group was obviously decreased (P<0.01), but PGC-1α mRNA expression increased with no statistical difference (P＞0.05). Conclusion The kidney-tonifying, spleen-strengthening, and blood stasis removing recipe enhances rat BMD by increasing the expression of PGC-1α mRNA and decreasing the expression of ERRα mRNA. |
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