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| β-TCP修复骨质疏松性骨缺损的实验研究 |
| Experimental study on the repairing of osteoporotic bone defect using β-TCP |
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| DOI：10.3969/j.issn.1006-7108.2014.10.008 |
| 中文关键词: 骨质疏松 骨缺损 β-TCP 新生骨 |
| 英文关键词:Osteoporosis Bone defect β-TCP New bone |
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| 中文摘要: |
| 目的 探索β-TCP修复骨质疏松性大鼠骨缺损的效果。方法 取SD大鼠20只，先去卵巢建立骨质疏松模型，等饲养3个月保证骨质疏松模型成功后再建立双侧大鼠股骨干骺端缺损模型，将手术成功的骨质疏松性骨缺损模型随机均分为两组，β-TCP植入组于骨缺损处植入β-TCP，空白对照组不作任何处理。术后4，8周进行大体观察、X射线摄片、组织病理学观察，Micro-CT观察。结果 术后4周、8周，β-TCP 植入组：缺损区X射线影像有明显的骨组织生成，HE染色可见大量骨小梁和较多核深染的长梭形骨细胞，Micro-CT显示骨微观结构修复效果较好，且能明显增加股骨骨密度；空白对照组X射线骨组织生成较少，骨小梁或骨板结构较少，排列较紊乱，Micro-CT显示骨微观结构修复效果不够理想，股骨骨密度增加不明显。结论 β-TCP可较好地修复骨质疏松性大鼠股骨干骺端缺损。 |
| 英文摘要: |
| Objective To investigate the role of β-TCP in bone defect repairing in osteoporotic rats. Methods Twenty SD rats were ovariectomized and fed for 3 months to establish the osteoporosis model. Subsequently, bone defects were created in bilateral femoral metaphysis by surgery. Successful osteoporosis bone defect models were divided into 2 groups, including β-TCP group and control group. β-TCP was embedded into bone defect in β-TCP group. No treatment was performed in control group. Gross observation, X-ray radiographs, tissue pathological observation, and Micro-CT observation were conducted 4 and 8 weeks after the surgery. Results X-ray radiographs showed that bone tissue growth was obvious in bone defect zone 4 weeks and 8 weeks after surgery in β-TCP group. HE staining showed that there were considerable amount of trabeculae and spindle shaped, deep stained osteoblasts. Micro-CT showed that bone micro-structure was comparatively well repaired, and the bone mineral density of the femur increased significantly. X-ray radiographs showed few bone growth, few trabeculae or bone lamella structure, and irregular distribution in control group. Micro-CT indicated that bone micro-structure was not desirably repaired, and the increase of bone mineral density of the femoral was not obvious. Conclusion β-TCP can comparatively well repair femoral metaphysis bone defect in osteoporotic rats. |
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