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| 铁调素对小鼠单核细胞RAW264.7膜铁转运蛋白1（FPN1）表达的影响 |
| Effect of hepcidin on the expression of ferroportin 1 in mouse RAW264.7 cells. |
| 投稿时间：2014-02-05 |
| DOI： |
| 中文关键词: 铁调素 RAW264.7细胞 膜铁转运蛋白 1 |
| 英文关键词:Hepcidin RAW264.7 cells Ferroportin 1 |
| 基金项目:国家自然科学基金(81273090)；江苏省自然科学基金(BK2012608)；苏州市科技基础设施建设计划之高技术研究重点实验室资助项目(SZS2012208)；苏州市科学技术项目(SS201327)；江苏省研究生创新工程项目(CX2213＿0834) |
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| 中文摘要: |
| 目的：观察铁调素对小鼠单核细胞RAW264．7膜铁转运蛋白1（ FPN1）的表达，探讨铁调素对RAW264．7细胞作用的可能通路和机制。方法将不同浓度的铁调素加入含有核因子κB受体活化因子配体（ RANKL）的RAW264．7细胞培养基，24 h后用免疫荧光和Western－blot方法测定FPN1的表达，共聚焦显微镜（ CLSM ）测定细胞内铁离子的浓度。结果 RAW264．7细胞膜上存在FPN1受体的阳性表达；在本实验铁调素浓度干预范围内，FPN1的表达随着铁调素浓度的增加呈浓度依赖性降低（P＜0．05），同时细胞内铁离子的含量随着铁调素浓度的增加呈浓度依赖性增加（P＜0．05）。结论小鼠单核细胞RAW264．7是铁调素作用的靶细胞，铁调素可通过降解其细胞膜上的FPN1增加细胞内的铁离子。 |
| 英文摘要: |
| Objective To investigate the effect of hepcidin on the expression of ferroportin 1 ( FPN1 ) in RAW264.7 cells in vitro, and to explore the possible mechanism.Methods Mouse monocytes RAW264.7 were treated with different concentrations of hepcidin in the presence of receptor activator of NF-Kb ligand ( RANKL) .After 24 hours, the expression of FPN1 was detected using Western blotting and immunofluorescence, respectively.The intracellular iron concentration was measured using a confocal laser scanning microscope.Results The results documented the existence of FPN1 at the membrane of RAW264.7 cells, and the expression of FPN1 was markedly downoregulated by hepcidin in a concentration-dependent manner ( p <0.05 ) .Furthermore, hepcidin increased intracellular iron in RAW264.7 cells in a concentration-dependent manner (p<0.05).Conclusion RAW264.7 cells are target cells of hepcidin.Hepcidin can increase intracellular iron by degradation of FPN1 at the membrane of RAW264.7 cells. |
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