| Objective To compare the three low concentrationss of medical ozone for treating knee osteoarthritis ( OA) through intra-articular injection using the established knee OA model in dogs.Methods Forty healthy male dogs were randomly divided into Group A ( the air group) , Group B ( the 10 ug/ml medical ozone group) , Group C ( the 20 ug/ml medical ozone group) , and group D (the 30 ug/ml medical ozone group), with 10 dogs in each group.The left knee joints were selected as Group N (the normal control group) .Hulth method was used to create OA model.The range of knee motion was measured before modeling, successful modeling, and before execution of the dogs.Nitrale reduetase ( NR) method was used to detect the concentration of TNF-a in the joints.Results The range of knee motion in Group A decreased obviously after 8 weeks.The increased was more in Group B, C, and D after the therapy than that at the successful modeling.The range of knee motion in Group C increased more obviously than that in Group B and D (P<0.05).Articular cartilage pathological damage was found in the right knees of the dogs in 4 groups, and the most serious cartilage lesion was in Group A and the lest in group C.TNF-a concentration in Group C decrased more obviously than that in Group A, B, and D, which was of statistical difference ( P<0.05 ) .Conclusion The 20 ug/ml medical ozone can reduce inflammatory reaction, enhance the ability to eliminate free radicals of the body, and inhibit the expression of TNF-a. |