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| 补肾中药血清联合BMP-2对人脐血间充质干细胞 增殖及形态学的影响 |
| Effect of the combination of kidney-tonifying Chinese herb containing serum and BMP-2 on the proliferation and morphology of human mesenchymal stem cells derived from umbilical cord blood |
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| DOI： |
| 中文关键词: 中医中药 补肾中药 BMP-2 ；脐血间充质干细胞 细胞增殖 |
| 英文关键词: |
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| 中文摘要: |
| 目的观察补肾中药血清联合BMP-2对人脐血间充质干细胞（UCB-MSCs)增殖及形态学的影响，并探讨其最佳时效。 方法倒置显微镜观察原代、传代培养及干预后UCB-MSCs的形态学变化;取第3代UCB-MSCs，随机分为8组，给予不同浓度 补肾中药血清及腿P-2条件培养基培养，分别于诱导后1 d、3 d、5 d采用MTT法检测各组细胞增殖情况。结果原代培养 UCB-MSCs随时间延长，数量逐渐增多且逐渐变长，2周时多呈长梭形、旋涡状生长，传代培养3 d即达到70% ~ 80%融合，以 梭形为主，符合干细胞的生长特征;干预后第3、5 d，5%、10%中药+ BMP-2组和BMP-2组均能够促进UCB-MSCs增值（P <05)，且5%中药+ BMP-2组优于BMP-2组（P = 0. 041)，增殖髙峰出现在干预后第3 d;经条件培养3 d后，各组细胞均可见 由长梭形变成立方形或多角形，尤以5%中药联合腿P-2组及腿P-2组较为明显。结论补肾中药血清联合BMP-2可协同 促进体外培养UCB-MSCs增殖，增殖效应与干预时间和中药血清浓度有一定相关性。 |
| 英文摘要: |
| Objective To observe the effect of kidney-tonifying Chinese herb containing serum combined with BMP-2 on the morphology and proliferation of human umbilical cord blood mesenchymal stem cells (UCB-MSCs). Methods The morphology change of primary’ passaged，and intervened UCB-MSCs was observed using an inverted microscope. The third passage of UCB- MSCs was randomly divided into 8 groups culturing in different concentrations of kidney-tonifying Chinese herb containing serum and BMP-2. The cell proliferation was detected using MTT method at 1-，3-，and 5-day after the intervention. Results The number of primary UCB-MSCs increased and the shape enlarged gradually along with the culturing time. After 2 weeks， most of the UCB-MSCs were characterized by spiral shape growth. In 3 days after passage’ 70% - 80% of the cells fused in the culture’ which met the growth characteristics of the stem cells. The cell proliferation in 5% and 10% combining groups and BMP-2 group increased after 3-and 5-day intervention (P <0. 05). The effect in 5% combining group was superior to that in BMP-2 group (P =0. 041). The proliferation peak appeared on the third day after the intervention. The UCB-MSCs became cubical or polygonal shape in 3 days |
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