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| 铁蓄积小鼠去势前后骨吸收活性的差异 |
| Variations in bone resorption activity between normal and ovariectomized mice with iron accumulation |
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| DOI： |
| 中文关键词: 雌激素 去卵巢 铁蓄积 骨吸收 |
| 英文关键词: |
| 基金项目: |
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| 摘要点击次数: 988 |
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| 中文摘要: |
| 目的了解小鼠在去势前与去势后，铁对骨吸收影响的差异。方法建立髙铁小鼠模型，将小鼠随机分为假手术组 (SHAM组）、髙铁去势组（F + OVX组）、去势组（OVX组）。铁剂干预两周后去势。在去势前（雌激素存在）与去势7周后（雌 激素缺乏）检测小鼠体重，血清铁蛋白;反转录聚合酶链反应（RT-PCR)检测胫骨内抗酒石酸酸性磷酸酶（TRAP)、降钙素受体 (CTR)、基质金属蛋白酶9(MMP9)、组织蛋白酶K(cathepsin K)、雌激素受体a(ERa)、雌激素受体P(ERP)表达情况，股骨远 端MioCT三维分析，提取各组小鼠股骨骨髓细胞进行破骨细胞培养，抗酒石酸酸性磷酸酶（TRAP)染色观察破骨细胞分化 情况。结果各组小鼠体重无明显差异。F + OVX组铁蛋白含量明显升髙（P <0.05)。Micro-CT分析表明:雌激素存在时，正 常组与铁干预组骨密度及相关指标无明显差异（P >0.05)；雌激素缺乏时，与OVX组比较，F + OVX组骨量下降更为严重（P <0.05)。半定量PCR结果显示:雌激素存在时，两组TRAP、CTR、MMP9、cathepsin K基因表达无统计学差异;雌激素缺乏时， F + OVX组各基因表达水平较OVX组显著升髙（P <0. 05)。去势后ERP表达下降（P <0. 05)，ERa无明显差异。TRAP染色 显示去势前各组小鼠破骨细胞数无明显差异（P >0. 05);而去势后，F + OVX组破骨细胞数明显髙于OVX组（P <0.05)。结 论未去势时（有雌激素），FAC对骨吸收影响甚微;去势后（无雌激素），FAC能显著增强破骨活性。 |
| 英文摘要: |
| Objective To explore the effect of iron on bone resorption before and after ovariectomy in mice. Methods A mouse model of high iron accumulation was established. The mice were randomly divided into SHAM group，OVX group，and F + OVX group. After 2-week iron intervention，the mice were ovariectomized. Body weight of the mice was examined before and after ovariectomy. Serum ferritin (Fer)，gene expression of TRAP，CTR，MMP9，cathepsin K，ERa，and ERp were detected. The distal femur was analyzed using 3-D micro-CT. Bone marrow-derived cells were extracted from the femurs of each group for osteoclast culturing. Tartrate resistant acid phosphatase (TRAP) staining was performed to observe the osteoclast differentiation. Results Body weight of the mice between each group was not significantly different. The serum Fer was significantly high in F + OVX group (P <0. 05). Micro-CT analysis indicated that BMD and other parameters were not different between SHAM group and iron-treated group in the presence of estrogen (P >0.05). However，BMD in F + OVX group was much lower than that in OVX group (P <0.05). Semi-quantitative RT-PCR showed that the expressions of TRAP，CTR，MMP9，and cathepsin K were not significantly different between the 2 groups in the presence of estrogen. The expressions was increased significantly in F + OVX group than in OVX group (P < 0. 05). ERp significantly decreased after ovariotomy，while the expression of ERa showed no |
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