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| 巴戟天含药血清对成骨-破骨细胞共育体系 OPGmRNA、RANKLmRNA 表达的影响 |
| Effect of morinda officinalis- containing serum on the mRNA expressions of OPG and RANKL in the osteoblast and osteoclast co-culture system |
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| DOI： |
| 中文关键词: 中医中药；巴戟天；成骨-破骨细胞共育体系；细胞核因子kB受体活化因子配体（RANKL) 破骨细胞抑制因子 (OPG，osteoprotegerin) 细胞核因子 kB 受体活化因子（RANK，receptor activator of NF-kB) |
| 英文关键词:Chinese medcine and Chinese herb Morinda officinalis Osteoblast and osteoclast co-cultured system RANKL OPG |
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| 中文摘要: |
| 目的探讨不同浓度巴戟天含药血清对体外成骨-破骨细胞共育体系OPG、RANKLmRNA表达的影响方法取健康5 周龄SD大鼠12只，体重165 -172 g。随机分4组，分别予生理盐水及低中髙三种浓度巴戟天溶液灌胃，分离血清。取健康5 周龄SD大鼠6只，体重165 - 172 g，取四肢长骨骨髓基质细胞，诱导培养破骨细胞;用24 h内新生SD乳鼠4只，体重5 ~6 g， 头盖骨分离体外培养成骨细胞。将成骨细胞加人含有培养5 d的破骨细胞中，共育2 d后分别改用不同浓度巴戟天含药血清 与不含药血清的培养基培养，设低、中、髙浓度组及对照组，培养3d后提取各组总RNA，应用RT-PCR检测各组OPGmRNA、 RANKLmRNA表达。结果RT-PCR结果示，中、髙浓度组RANKLmRNA表达量均低于对照组（P < 0. 05)，而OPGmRNA表达 量显著髙于对照组(P <0. 001)。结论巴戟天含药血清可上调共育体系中OPGmRNA表达，并下调RANKLmRNA表达，从而 降低破骨细胞分化成熟及骨吸收活性。 |
| 英文摘要: |
| Objective To investigate the effect of the morinda officinalis-containing serum on the mRNA expressions of the OPG and RANKL in osteoblast and osteoclast co-culture system. Methods Twelve 5-week-old healthy SD rats (165 - 172g) were randomly assigned to 4 groups. Rats in each group were given different concentrations of morinda officinalis-containing serum or normal saline. The blood of the animals was extracted. Bone marrow cells were harvested from the femurs and tibiae of 6 5-week- old SD rats (165 - 172g). The cells were induced to osteoclasts. Osteoblasts were separated from the cranium of 4 24h newborn SD rats (5 - 6g). The osteoblasts were added to 5-day osteoclast culture. After 2-day co-culture ’ the different concentrations of morinda officinalis-containing serum or normal saline-containing serum were added to the culture plates. After intervention 3 days， total RNA was extracted from cells in high， middle， and low concentrations of morinda officinalis groups and control groups. The OPG and RANKL mRNA expressions were detected using real-time PCR. Results The results of RT-PCR indicated that RANKL mRNA expression was lower in all concentrations of morinda officinalis-containing serum groups than in the control group (P < 0.05)，but OPG expression was higher than in the control group (P <0. 001). Conclusion Morinda officinalis-containing serum up-regulates the expression of OPG mRNA ’ but down-regulates the expression of RANKL mRNA in the co-culture system ’ leading to the decrease of osteoclast differentiation and activity of bone resorption. |
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