中国骨质疏松杂志

司坦唑醇预防维甲酸致大鼠骨损害的作用

Preventive effect of stanozolol on retinoic acid-induced bone lesion in rats

DOI：10.3969/j.issn.1006.7108.2015.03.008

英文关键词:Osteroporosis Retinoic acid Stanozolol BMD Biomechanical properties Bone histomorphometry

基金项目:国家自然科学基金资助项目（81373499);广东省科技计划项目（2011B060300032，2010A040200005);广东医学院科技创新团队基金（STIF201104 )

作者	单位
陈秋生1 陈文双2 张雪梅3 张新乐3 吴铁3 崔燎2 吴科锋2 许碧莲3*	1. 广东医学院生理科学实验室，湛江524023 2. 科研中心 3. 药理教研室

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中文摘要:

目的利用骨密度、骨生物力学、骨组织形态计量学等方法探讨司坦唑醇对维甲酸致骨损害的预防作用。方法 4月龄的SPF级雌性SD大鼠24只，随机分成正常对照组、维甲酸组、司坦唑醇组。正常对照组给予溶剂对照溶液，维甲酸组给予维甲酸70 mg ?kg-1?d-1，司坦唑醇组给予维甲酸70 mg? kg-1?d-1和司坦唑醇0.5 mg? kg-1?d-1。大鼠每次均按5 ml ?kg-1灌胃给药，连续给药28天。实验结束后取大鼠右侧股骨进行骨密度和生物力学参数测量，取右侧胫骨上段和中段进行骨组织形态计量学测量。结果与正常对照组比较，维甲酸组大鼠股骨骨密度降低10. 2% (P <0. 01)，生物力学参数弹性载荷、最大载荷、断裂载荷、刚性系数、弯曲能量分别降低39. 1%、32. 9%、36. 4%、40. 2%、38. 0%( P <0. 01)，胫骨上段松质骨静态参数骨组织总面积、骨小梁面积、骨小梁周长分别降低31. 6%、40. 0%、36. 2% (P <0. 01)，动态参数标记周长百分数(％ L. Pm)、骨形成率(BFR/TV、BFR/ BS、BFR/BV)分别降低57. 1%、69. 9%、62. 0%、65. 8% (P <0.01)。胫骨中段皮质骨静态参数（骨组织总面积、皮质骨面积)和骨外膜动态参数均降低，而骨内膜动态参数增强。与维甲酸组比较，司坦唑醇组股骨弹性载荷、刚性系数、弯曲能量分别增加22. 2%、22. 9%、17. 7%(P <0. 05)，而骨密度、胫骨上段松质骨和中段骨皮质骨组织形态计量学参数均无明显变化。结论司坦唑醇可部分改善维甲酸所致大鼠骨损害。

英文摘要:

Objective To investigate the preventive effect of stanozolol on retinoic acid-induced bone lesion in rats using bone mineral density (BMD)，biomechanics，and bone histomorphometry methods. Methods Twenty-four 4-month-old virgin female Sprague-Dawley rats were randomly divided into control group，retinoic acid (RA) group，and stanozolol group. Rats in control group were given vehicle. Rats in RA groups were given RA of 70 mg? kg-1?d-1. Rats in stanozolol group were given RA of 70 mg? kg-1?d-1 and stanozolol of 0. 5 mg? kg-1?d-1. All the rats were oral gavaged at 5 mL? kg 1 for 28 days. At the experimental endpoint，bone mineral density (BMD) of the right femur was examined，and biomechanical properties and bone histomorphometric parameters of the proximal tibial metaphysis (PTM) and the tibial shaft (Tx) were measured. Results Compared to those in control group，BMD decreased by 10. 2%，and biomechanical properties including elastic load，maximum load，break load，rigid coefficient，and bending energy decreased by 39. 1%，32. 9%，36. 4%，40. 2%，38. 0% in femur in RA group，respectively (P <0.01). Compared to those in control group，the static parameters including total tissue area，trabecular area，and trabecular perimeter decreased by 31. 6%，40. 0%，and 36. 2% (P <0. 01)，and the dynamic parameters including percent labeled perimeter (% L. Pm)，bone formation rate (BFR/TV，BFR/BV，and BFR/BS) decreased by 57. 1%，69. 9%，62. 0%，and 65. 8%，respectively, in PTM in RA group (P < 0. 01). Compared to those in control group, the static parameters (the total tissue area and cortical area) and the dynamic parameters of periosteum of Tx significantly decreased，while the dynamic parameters of endosteum of Tx significantly increased in RA group. Compared to those in RA group， the elastic load， rigid coefficient，and bending-energy increased by 22. 2%，22. 9%，and 17. 7%，respectively，in the femurs in stanozolol group. No significant changes of BMD and histomorphometric parameters in PTM and Tx were found in stanozolol group. Conclusion Stanozolol can partially relieve bone damage induced by RA in rats.

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