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| 长期使用糖皮质激素对大鼠的骨生物力学影响 |
| The effect of long-term use of glucocorticoids on bone biomechanics of rats |
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| DOI：10.3969/j.issn.1006.7108.2015.04.007 |
| 中文关键词: 糖皮质激素性骨质疏松 大鼠 骨生物力学 |
| 英文关键词:Osteoporosis |
| 基金项目:广东省科技计划项目（2010B031600291，2011 B031800229) |
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| 中文摘要: |
| 目的 探讨长期使用糖皮质激素对大鼠的骨质量影响。方法 3月龄SD大鼠共38只，雌性。随机分成4组，分别是： (1)基础对照组(B组，8只，实验开始时处死，建立本批大鼠底线）；（2)正常对照组（C组，10只，腹腔注射生理盐水75 mg/ kg);(3)低剂量激素组(L组，10只，腹腔注射甲强龙50 mg/kg) ;(4)高剂量激素组（H组，10只，腹腔注射甲强龙75 mg/kg)。 实验开始时，低剂量激素组和高剂量激素组连续使用甲强龙3天(75 mg/kg. d)，停用一周后，按计划使用甲强龙，每周一次，用药24周后取股骨和第三腰椎行骨生物力学实验。结果（1)与正常对照组相比，低剂量激素组和高剂量激素组的实验后体重明显减少(P <0.05);(2)与正常对照组相比，低剂量激素组和高剂量激素组股骨的结构力学参数（载荷、刚性）和材料力学参数(破断强度、刚性模量）均显著降低(P <0. 05)；(3)与正常对照组相比，低剂量激素组和高剂量激素组腰椎的材料力学参数(破断强度、刚性模量）均显著降低(P <0. 05)。结论 长期使用糖皮质激素激素，无论剂量高低，均会导致大鼠股骨和腰椎骨质量下降。 |
| 英文摘要: |
| Objective To determine the effect of long-term use of methylprednisolone on bone biomechanics of rats. Methods Thirty-eight 3 -month-old female SD rats were chosen. They were randomly divided into basal control group (B group，8 rats were sacrificed at the beginning of the experiment as baseline)，normal control group ( C group， 10 rats were given intraperitoneal injection of 75mg/kg normal saline)，low dose methylprednisolone group(L group，10 rats were given intraperitoneal injection of 50 mg/kg methylprednisolone)，and high dose methylprednisolone groug (H group，10 rats were given intraperitoneal injection of 75 mg/kg methylprednisolone). Methylprednisolone was given in L group and H group for 3 days，and stopped for a week. Then the intraperitoneal injection was given once a week according to the plan. After 24 weeks of the treatment，the femurs and the lumbar spine L3 were collected for biomechanical experiment. Results (1) Compared to that in C group，the body weight in L group and H group decreased obviously after the experiment (P <0. 05). (2) Compared to those in C group，structural mechanical parameters ( load and rigidity) and material mechanical parameters ( breaking strength and modulus of rigidity) of the femurs in L group and H group significantly decreased (P<0. 05). (3) Compared to those in C group，structural mechanical parameters (load and rigidity) and material mechanical parameters ( breaking strength and modulus of rigidity) of the L3 in L group and H group significantly decreased (P <0.05). Conclusion The long-term use of methylprednisolone，regardless of dose，results in the decrease of bone quality of the femur and the lumbar spine of rats. |
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