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| lncRNA在绝经后骨质疏松症肾阴虚证中的表达特征 及调控网络分析 |
| Analysis of lncRNA expression and regulation network in postmenopausal osteoporosis patients with Kidney Yin deficiency |
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| DOI： |
| 中文关键词: 中西医结合 肾阴虚证 绝经后骨质疏松症 lncRNA 基因芯片 调控网络 |
| 英文关键词: |
| 基金项目:福建省中医药研究院自主选题项目（2013fjzyyk-4)、福建省科技厅省属公益类科研院所基本科研专项（2014R1035-15);福建 省卫生厅青年科研课题（2013 -2 -73);福建省教育厅青年科研课题（JB13093) |
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| 中文摘要: |
| 目的探讨绝经后骨质疏松症肾阴虚证中lncRNAs的表达特征及基因调控网络。方法随机选择绝经后骨质疏松症 受试者，分为肾阴虚证组3例和肾阳虚证组3例，并选择健康绝经后妇女3例为正常对照组，采用基因芯片技术检测外周血单 个核细胞lncRNAs的表达水平。肾阴虚证组分别与其他2组比较，筛选共同差异表达lncRNAs，并构建lncRNA调控网络;实 时荧光定量PCR检测LINC00334、LINC00189和LOC101929378的表达，验证基因芯片结果。结果肾阴虚证组与正常对照组 和肾阳虚证组比较，筛选出 8 个共同差异表达 lncRNAs： LINC00334、LINC00189、L0C101929378、XLOC _013921、 ENSG00000237489. 2、ENSG00000225278. 2、AK125437 和 RNA95672;这些差异 lncRNAs 参与 Jak/STAT、MAPK、胰岛素通路和 钙离子代谢等12条信号转导通路的调控;实时荧光定量PCR证实，与正常对照组比较，LINC00189在绝经后骨质疏松症肾阴 虚证表达下调（FC =4. 71，P =0. 007) ；LINC00334(FC =6. 83，P =0. 005)和L0C1019293"78绝经后骨质疏松症肾阴虚证表达上 调（FC = 4.51，P = 0.035 )，变化趋势与芯片结果相一致。结论 LINC00334、LINC00189、L0C101929378、XL0C_013921、 AK125437、ENSG00000237489. 2、ENSG00000225278. 2 和 RNA95672 等 8 条 lncRNAs 可能通过调控 Jak/STAT、MAPK、胰岛素通 路和钙离子代谢等信号通路参与绝经后骨质疏松症肾阴虚证的发生发展过程。 |
| 英文摘要: |
| Objective To investigate the lncRNA expression and regulation network in postmenopausal osteoporosis with kidney Yin deficiency. Methods The patients with postmenopausal osteoporosis were selected and randomly divided into ： kidney Yin deficiency group (n = 3) and kidney Yang deficiency group (n = 3). Three healthy postmenopausal women were selected as control group. lncRNA gene expression in single nuclear cells in peripheral blood was examined using microarray method. The genes in kidney Yin deficiency group were compared to those in other 2 groups respectively. Common differential expressed genes were studied and the regulation network was established. Real-time RT-PCR was used to examine the expression of LINC00334， LINC00189，and LOC101929378 and the results of microarray were confirmed. Results Eight differential expressed lncRNAs， LINC00334 LINC00189，LOC101929378，XLOC —013921，ENSG00000237489.2，ENSG00000225278.2，AK125437，and RNA95672 were screened out from the comparison among the 3 groups. Pathway analysis indicated that 12 potential pathways corresponded to these aberrant lincRNAs. Real-time PCR confirmed that LINC00189 was down—egulated in the kidney Yin deficiency group (FC =4.71，P = 0. 007). LINC00334 (FC =6. 83，P = 0. 005 ) and LOC101929378 (FC =4.51，P = 0. 035) were up—egulated in the kidney Yin deficiency group. The change trend was coincident with the result of CHIP. |
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