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| 橄榄苦苷对成骨细胞OPG/RANKL mRNA的影响 |
| Effects of oleuropein on OPG/RANKL mRNA expression in osteoblasts |
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| DOI：10.3969/j.issn.1006-7108.2015.06.002 |
| 中文关键词: 橄榄苦苷 成骨细胞 OPG/RANKL mRNA 影响 |
| 英文关键词:Oleuropein Osteoblasts OPG/RANKLmRNA Influence |
| 基金项目:军区医学科技创新课题（11M111） |
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| 中文摘要: |
| 目的 通过测定橄榄苦苷对成骨细胞OPG/RANKL mRNA的表达，探讨其治疗骨质疏松症的机制。方法 运用荧光定量PCR技术（RT-PCR）检测不同浓度橄榄苦苷不同时间对成骨细胞OPG/RANKL mRNA 表达量。结果 与阴性对照组比较，RT-PCR 结果显示不同浓度的橄榄苦苷均能促进OPG mRNA的表达，并且抑制成骨细胞RANKL mRNA 的表达。结论 橄榄苦苷可能通过抑制RANKL分泌来降低破骨细胞活性，同时促进成骨细胞分泌OPG，使之与RANKL结合增多，间接作用于破骨细胞，使其活性降低，而达到治疗骨质疏松的目的。 |
| 英文摘要: |
| Objective To explore the mechanism of oleuropein in the treatment of osteoporosis by measuring the expression of OPG/RANKL mRNA in osteoblasts. Methods The expression of OPG/RANKL mRNA in osteoblasts was detected using fluorescence quantitative PCR (RT-PCR) in the presence of different solubility of oleuropein at different time. Results Compared to the control group, the results of RT-PCR showed that oleuropein of all concentrations promoted the expression of OPG mRNA, and inhibited the expression of RANKL mRNA. Conclusion Oleuropein reduces the activity of osteoclasts by inhibiting the secretion of RANKL, and simultaneously promotes osteoblasts to secrete OPG, which combines with RANKL that indirectly affects osteoclasts, leading to the decrease of osteoclast activity and reaching the purpose of the treatment of osteoporosis. |
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