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| 低强度脉冲超声对骨质疏松症小鼠模型的作用 |
| Effects of low-intensity pulsed ultrasound on mouse osteoporosis model |
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| DOI：10.3969/j.issn.1006-7108.2015.06.004 |
| 中文关键词: 低强度脉冲超声 骨质疏松症 骨密度 卵巢切除术 雌激素 |
| 英文关键词:Low-intensity pulsed ultrasound Osteoporosis Bone mineral density Ovariectomy Estrogen |
| 基金项目:北京市科技计划资助项目(Z111100067311053) |
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| 中文摘要: |
| 目的 低强度脉冲超声具有促进成骨细胞增殖和矿化的作用，本实验在以往细胞实验的基础上，探究一定参数的超声对骨质疏松症小鼠模型的作用。方法 采用8周龄SPF级BalB/C雌性小鼠，对其实施双侧卵巢切除术建立小鼠骨质疏松症模型，并确定建模成功。实验设置空白对照组、阳性对照组（雌激素灌胃）和超声实验组。阳性对照组给予含结合雌激素（1.24μg/只）的超纯水溶液灌胃。超声组选用频率为1.5MHz强度为40mW/cm2的超声照射脊柱5min，每周5次，持续8w后检测脊柱区域骨组织密度和生物力学性能。结果 建模后小鼠血清中雌二醇含量降低，并且骨密度明显下降。经过后续干预治疗后，雌激素治疗组和超声组骨密度有一定提高。生物力学测试结果显示雌激素组和超声组较对照组而言，脊柱最大载荷和最大位移均有所提高。实验后对模型小鼠五脏进行病理切片，未见血点，细胞结构完整。结论 本实验成功建立了小鼠骨质疏松症模型，低强度脉冲超声能够提高骨质疏松症小鼠模型的骨密度，增加骨强度和骨韧性且该参数的超声具有良好的生物安全性。 |
| 英文摘要: |
| Objective Low-intensity pulsed ultrasound (LIPUS) promotes osteoblast proliferation and mineralization. This experiment explores the effect of LIPUS on mouse osteoporosis model on the basis of our previous cell experiments. Methods Eight-week-old healthy female BalB/C mice (SPF grade) were ovariectomized bilaterally to establish the mouse osteoporosis model. After the models were established, mice were divided into blank control (CON), positive control group (estrogen group, ESG), and LIPUS group. The mice were gavaged 1.24μg estrogen containing distilled water each in the ESG. The mice were irradiated on the spine using ultrasound (1.5MHz, 40mW/cm2) for 5 minutes in the LIPUS group. Each mouse was treated 5 times per week for 8 weeks. Eight weeks later, bone mineral density and biomechanical properties around the irradiated spine were measured. Results Serum estradiol and bone mineral density decreases obviously after establishing the osteoporosis model in mice. After the follow-up intervention treatment, bone mineral density increased in a certain extent in LIUPS and ESG. Biomechanics detection showed that the maximum load and maximum displacement improved in ESG and LIUPS compared with those in CON. No bleed points were observed and the cell structure was intact in organ histological section after the experiments. Conclusion We successfully establish the mouse model of osteoporosis. The results show that low-intensity pulsed ultrasound is a safe method that can improve bone mineral density in osteoporotic mice and enhance bone strength and bone tenacity. |
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