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| 特立帕肽对去势大鼠骨缺损修复影响的实验研究 |
| The effect of teriparatide on the bone defect healing in ovariectomized rats |
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| DOI：10.3969/j.issn.1006-7108.2015.06.007 |
| 中文关键词: 骨质疏松 骨缺损 PTH Micro-CT |
| 英文关键词:Osteoporosis Bone defect PTH Micro-CT |
| 基金项目:2011年浙江省高校重中之重学科（外科学）开放基金（2011GK006） |
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| 中文摘要: |
| 目的 探索特立帕肽（PTH）对去势大鼠骨缺损修复的影响。方法 选取SPF级3月龄雌性SD大鼠20只，行双侧卵巢切除术,饲养3月后骨质疏松动物模型成功建立，然后于大鼠两侧股骨髁上行直径3mm贯通性骨缺损手术，术后随机分为对照组和PTH治疗组，PTH治疗组于缺损手术1w后，每周给予背部皮下注射特立帕肽5次（30μg/kg），空白对照组注射等量的生理盐水。术后4，8w进行组织病理学观察，Micro-CT分析检测。结果 术后4、8w，PTH治疗组和对照组对比发现：PTH治疗组股骨骨密度均高于同时间段的对照组，缺损区Micro-CT扫描显示PTH治疗组较对照组有更为明显的新生骨组织形成，骨缺损术后4、8w时，两组大鼠股骨干骺端骨缺损区域骨小梁体积分数（BV/TV）、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)均逐渐增加，骨小梁分离度(Tb.Sp)降低， PTH治疗组BV/TV、Tb.Th及Tb.N均较同时期的空白对照组增高，骨小梁分离度(Tb.Sp)则低于空白对照组，差异有统计学意义(P<0.05)。缺损术后8w时PTH治疗组骨缺损区域可见大量排列致密且形态粗大的骨小梁，骨缺损区域基本愈合，而对照组缺损区域愈合欠佳。结论 PTH可较好地促进骨质疏松性大鼠股骨干骺端骨缺损的修复。 |
| 英文摘要: |
| Objective To explore the effect of teriparatide (PTH) on bone defect healing in ovariectomized rats. Methods Twenty SPF grade 3-month-old SD rats were selected and ovariectomized, and fed for 3 months to establish the osteoporosis model. Then a 3mm femur defect drilling was created in bilateral femoral metaphysis of the rats. After the operation, rats were divided into control group and PTH-treated group. After one week, rats in PTH-treated group received subcutaneous PTH injection five times (30ug/kg), while rats in control group received saline injection. Histological studies and mirco-CT analysis were performed at 4 and 8 weeks after the surgery. Results Compared to that in control group, bone mineral density increased significantly in PTH-treated group after 4 and 8 weeks of operation. Micro-CT images of the defect showed that total bone volume/total tissue volume, cortical wall thickness, trabecular thickness, trabecular number increased significantly, and trabecular space decreased significantly in PTH-treated group compared to those in control group (P<0.05). In 8 weeks, large tense-arrayed and thick trabeculae could be seen in the defect area, and the defect was almost healed in PTH-treated group, while in control group the defect was not well healed. Conclusion PTH can promote femoral metaphases bone defect repair in osteoporotic rats. |
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