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| 25( OH) D3、维生素D受体与糖皮质激素性骨质疏松关系的研究 |
| Study on the relationship of VDR,25(OH)D3 and glucocorticoid-induced osteoporosis |
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| DOI：10.3969/j.issn.1006.7108.2015.07.001 |
| 中文关键词: 糖皮质激素 骨密度 VDR 25( OH) D3 |
| 英文关键词:Glucocorticoid Bone mineral density Vitamin D receptor 25(OH) D3 |
| 基金项目:国家自然科学基金（81260142);广西自然科学基金（2013GXNSFAA019174) |
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| 中文摘要: |
| 目的 观察地塞米松(Dex)不同作用时间对大鼠骨量、骨组织维生素D受体（VDR)和血清25羟维生素D3(25( OH) D3)的影响。方法 40只3.5月龄的sprague-dawley( SD)雌性大鼠，随机分为Dex组和对照组，每组20只，分别干预4周和9 周。Dex组肌肉注射Dex 2.5 mg/kg，每周2次，对照组注射等量生理盐水。干预4周和9周分别用双能X线骨密度仪测骨密度，逆转录聚合酶链反应和免疫组化法检测骨组织VDR mRNA和蛋白的表达水平，用ELISA法检测血清25( OH) D3的含量。 结果干预4周，Dex组大鼠体重下降(56. 67 ±24. 43) g，9周下降(85. 83 ±26. 35) g( P < 0. 05); Dex组体重低于对照组（P < 0.01)。干预9周，Dex组骨密度低于对照组(P < 0. 05)。干预4周，Dex组大鼠骨组织VDR mRNA表达高于对照组（1. 48 士 0. 32 vs 1. 15 ±0. 19)( P <0.05);9 周，Dex 组大鼠骨组织 VDR mRNA 表达低于对照组（1. 07 ± 0. 35 vs 1.38 ±0. 29) (P <0.05.。Dex组大鼠骨组织VDR蛋白表达在4周和9周与对照组均无差异(P >0.05)。干预4周，Dex组血清25( OH) D3含 量与对照组无差异（P >0.05);9周，Dex组血清25( OH) D3含量低于对照组（P <0.05)。结论 SD大鼠肌肉注射Dex 2.5mg/kg，每周2次，9周能成功建立糖皮质激素性骨质疏松模型。Dex可能通过减少VDR的转录和25( OH) D3的含量来引起骨密度下降，导致骨质疏松的发生。 |
| 英文摘要: |
| Objective To investigate the effect of dexamethasone (Dex) on bone mass，vitamin D receptor (VDR)，and 25 (OH) D3 in rats at different time. Methods Forty 3. 5 month-old Sprague Dawley ( SD) rats were randomly assigned to Dex group and control group，with 20 rats in each group. The rats in Dex group were intramuscularly injected 2. 5 mg/kg Dex twice a week，while rats in control group were injected equivalent normal saline intramuscularly. BMD was measured using dual energy X- ray absorptiometry. The expression of VDR mRNA and protein in bone tissue was measured with reverse transcription-olymerase chain reaction (RT-CR) and immunohistochemistry. 25-hydroxyvitamin D (25( OH) D3) was detected with ELISA. Results The weight of rats decreased 56. 67 ±24. 43g in 4 weeks after intervention，and decreased 85. 83 ±26. 35g in 9 weeks. The weight of rats in Dex group was lower than that in control group (P <0.01). At 4 weeks，the expression of VDR mRNA in Dex group was higher than that in control group (1. 48 ± 0. 32 vs 1. 15 ± 0. 19，P < 0. 05)，but it was lower at 9 weeks (1. 07 士 0. 35 vs 1. 38 ± 0. 29，P < 0. 05). The expression of VDR protein in Dex group was not different comparing to that in control group at 4 and 9 weeks. The serum level of 25 (OH) D3 in Dex group had no change at 4 weeks，but decreased significantly at 9 weeks (P < 0. 05). Conclusion GIOP animal model can be successfully established by injecting 2. 5mg/kg Dex twice a week intramuscularly for 9 weeks in SD rats. Dex may decrease BMD resulting in osteoporosis by decreasing VDR transcription and 25 (OH) D3 level. |
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