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| 锶盐对高浓度地塞米松作用下大鼠成骨细胞的影响 |
| Effect of strontium ranelate on rat osteoblasts under high dexamethasone concentration |
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| DOI：10.3969/j.issn.1006.7108.2015.07.009 |
| 中文关键词: 雷奈酸锶 地塞米松 大鼠 成骨细胞 |
| 英文关键词:Strontium ranelate Dexamethasone Rat Osteoblasts |
| 基金项目:2011年广东省医学科研基金项目（A2011317) |
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| 中文摘要: |
| 目的 探讨雷奈酸锶(Sr)对高浓度地塞米松(Dex)作用下大鼠成骨细胞（OB)的影响。方法 采用大鼠骨髓基质干细胞(BMSCs)来源的成骨细胞，运用MTT试剂盒和AKP试剂盒分别测定Sr对细胞增殖和分化的影响，定量PCR方法测定Sr对高浓度地塞米松作用下成骨钙素（OC)和转化生长因子-β(TGF-β)表达的影响。结果 Sr浓度在10-5、10-4 mol/L时，能够促进OB增殖和分化(P < 0. 01); Sr浓度为10-4mmol/L能够有效拮抗高浓度Dex( 10-5 mol/L)对成骨细胞分化抑制作用（P <0.01)，并能逆转Dex对成骨相关基因OC和TGF-β的抑制作用(P <0. 05，P <0. 01)。结论 Sr能够对大鼠BMSCs来源的成骨细胞的增殖、分化有促进作用，同时10-4 mol/L Sr可以拮抗Dex( 10-5 mol/L)对ALP和成骨相关基因的抑制作用。为Sr防治糖皮质激素性骨质疏松提供了细胞学依据。 |
| 英文摘要: |
| Objective To study the effect of strontium ranelate ( Sr) on rat osteoblasts under high dexamethasone (Dex) concentration. Methods Using rat bone marrow stromal stem cell-derived osteoblasts，the effect of Sr on the proliferation and differentiation of osteoblasts was detected with MTT and AKP commercial kits. The expression of OC and TGF-β under high Dex concentration was detected with RT-PCR. Results Proliferation and differentiation of OB were stimulated at concentrations of 10-5 mol/L and 10-4 mol/L of Sr (P < 0. 01). Sr at concentration of 10-4 mol/L effectively antagonized the inhibitory effect of Dex on osteoblast differentiation (P <0.01)，and reverted the inhibitory effect of Dex on OC and TGF-β(P < 0. 05，P < 0. 01). Conclusion Sr can promote the proliferation and differentiation of OB derived from BMSCs. Sr at concentration of 10-4 mol/L can effectively antagonize the inhibitory effect of Dex (10-5mol/L) on ALP，OC，and TGF-β. It provide the cytological evidence for prevention and treatment of GIOP with Sr. |
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