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| 绝经后妇女骨质疏松症肾阳虚证的关联蛋白LTBP1的表达及其cDNA测序的研究 |
| Analysis of the protein and cDNA expression of LTBP1 in postmenopausal osteoporosis patients with kidney Yang deficiency |
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| DOI：10.3969/j.issn.1006.7108.2015.08.002 |
| 中文关键词: 中医 绝经后骨质疏松症 肾阳虚证 LTBP1 ；蛋白表达 cDNA |
| 英文关键词:中医 绝经后骨质疏松症 肾阳虚证 LTBP1 蛋白表达 cDNA |
| 基金项目:国家自然科学基金（81173280);福建省卫生厅青年科研课题（2013-1-41);福建省中青年教师教育科研项目（JB13094)； 福建省省属公益类科研院所基本科研专项项目（2014R1035) |
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| 中文摘要: |
| 目的 探讨绝经后骨质疏松症肾阳虚证与LTBP1蛋白表达及cDNA变化的关联性。方法 随机选择绝经后骨质疏松症患者，中医辨证肾阳虚证组27例，27例健康绝经后妇女为对照组，Westeen -blot法检测外周血LTBP1的蛋白表达，对cDNA 片段进行测序。结果 肾阳虚证组LTBP1蛋白表达水平(0. 365 ±0. 278)低于健康对照组(0. 675 ±0. 583)(P <0. 05)，差异有统计学意义。cDNA测序在健康对照组与肾阳虚证组中碱基无有意义的突变。结论 绝经后骨质疏松症肾阳虚证与LTBP1蛋白表达下调相关联，测序发现与碱基突变无关。 |
| 英文摘要: |
| Objective To study the association between LTBP1 protein and cDNA expression and the kidney Yang deficiency syndrome in postmenopausal osteoporosis. Methods Patients with postmenopausal osteoporosis were randomly selected. Twenty- seven cases were in TCM syndrome of kidney Yang deficiency syndrome group，and 27 healthy postmenopausal women were in the control group. Protein expression of LTBP1 was detected using Western blotting method. The cDNA fragment was sequenced. Results LTBP1 protein expression level in kidney Yang deficiency syndrome group (0. 365 ±0. 278) was less than that in healthy controls (0. 675 ± 0. 583)，and the difference was statistically significant (P < 0. 05). There was no significant mutation in CDNA bases in both healthy controls and kidney Yang deficiency syndrome group. Conclusion The down-regulation of LTBP1 protein expression is associated with postmenopausal osteoporosis with kidney Yang deficiency syndrome. There is no correlation with mutation of DNA bases. |
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