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| 葛根素通过NF-κB通路抑制地塞米松诱导的 hFOB1. 19人成骨细胞凋亡 |
| Puerarin inhibits dexamethasone-induced apoptosis by hFOBl. 19 human osteoblast cells through NF-KB pathway |
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| DOI：10.3969/j.issn.1006.7108.2015.08.008 |
| 中文关键词: 葛根素 地塞米松 成骨细胞 凋亡 |
| 英文关键词:Puerarin Dexamethasone Osteoblast cells Apoptosis |
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| 中文摘要: |
| 目的 探索葛根素抑制地塞米松诱导的hFOB1.19人成骨细胞凋亡机制的实验研究。方法 MTS检测葛根素对 hFOB1. 19细胞增殖活性影响，免疫荧光检测DEX诱导hFOB1. 19细胞凋亡及葛根素抑制DEX诱导的细胞凋亡的细胞核改变。Western blot检测p-p65、p-IκB的蛋白表达情况。Realtime PCR检测Caspase-3、Caspase-9的基因表达情况。ELISA检测加人NF-kB抑制PDTC后对凋亡的影响。结果 10-8 M和10-9M葛根素明显增加细胞的增殖活性，10-5 M和10-6 M浓度的 DEX作用72h后诱导细胞凋亡，加人10-8M葛根素后细胞凋亡受到抑制。Western blot结果显示，加人DEX后，p-NF-κB4，p- IkB的表达上调，DEX和PUE共同处理后，DEX诱导的NF-κB和IkB的磷酸化受到抑制。Realtime PCR显示DEX处理后 caspase3、caspase8的mRNA基因表达明上调，而DEX和PUE共同作用后，caspase3、caspase8的mRNA基因表达下调。ELISA 显示PUE抑制DEX诱导的细胞凋亡。PDTC、DEX、PUE共同处理后，PUE对DEX诱导的细胞的保护作用受到部分抑制。结论 葛根素抑制地塞米松诱导的hFOB1. 19细胞凋亡通过依赖于Caspase调节NF-κB通路。 |
| 英文摘要: |
| Objective To explore the mechanism of puerarin in inhibition of dexamethasone4nduced apoptosis by hFOB1.19 cells. Methods The effect of puerarin on cell proliferation of hFOB1. 19 cells was detected using MTS method. The effect of dexamethasone on apoptosis and the protect effect of puerarin on hFOB1. 19 cells were detected using immunefluorescence method. The protein expression p-p65 and p-IKB was detected using Western blotting. The gene expression of caspase-3 and caspase-9 was detected using real-time PCR. The effect of NF-KB inhibitor PDTC on cells apoptosis was detected using ELISA method. Results Puerarin of 10- 8 M and 10- 9 M concentrations increased cell proliferation significantly. DEX of 10- 5 M and 10- 6 M concentrations increased cell apoptosis after 72h treatment. Puerarin of 10- 8 M concentration inhibited DEX-induced cell apoptosis. Western blotting results showed that the protein expression of p-65 and p-IkB increased after DEX treatment. DEX-induced phosphorylation of NF-KB and IKB was inhibited after addition of PUE. Real-time PCR showed that the gene expression of caspase3 and caspase8 mRNA was up-regulated after DEX treatment. This expression was down-regulated after addition of PUE. ELISA results showed that PUE inhibited the cells apoptosis induced by DEX. When additioned with PDTC，DEX，and PUE，the protective effect of PUE was inhibited partially. Conclusion Puerarin inhibits dexamethasone-induced hFOB1. 19 cells apoptosis through caspase regulation of NF-KB pathway. |
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