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| 糖尿病大鼠血管钙含量和骨密度变化的实验研究 |
| Experimental study of the changes of serum calcium content and bone mineral density in diabetic rats |
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| DOI：10.3969/j.issn.1006.7108.2015.11.006 |
| 中文关键词: 糖尿病 血管钙含量；骨密度 |
| 英文关键词:Diabetes Serum calcium content Bone mineral density |
| 基金项目:内蒙古自然科学基金资助项目(2014MS0345) |
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| 中文摘要: |
| 目的 观察糖尿病（diabetes mellitus，DM)大鼠模型血管钙含量和骨密度的变化。方法 用大剂量链脲佐菌素 (streptozotocin, STZ)—次性腹腔注射和高脂高糖饲料喂养加小剂量STZ注射制备1型和2型糖尿病大鼠模型,观察对血管钙含量和骨密度（bone mineral density ,BMD)的影响。雄性Wistar大鼠共32只随机分为4组（每组8只），即对照组、高脂组、1 型糖尿病组和2型糖尿病组。12周后，进行主动脉钙含量、碱性磷酸酶（alkaline phosphatase, ALP)活性和骨钙素（osteocalcin, OC)含量测定；用骨密度仪测定大鼠股骨和腰椎骨密度。结果 1型糖尿病组大鼠血管钙含量（71. 67± 10. 61μmol/mg. dw) 高于对照组和高脂组（44 ± 15. 87和44.8 ±9.44μmol/mg. dw) (P <0. 05)；2型糖尿病组大鼠血管钙含量（63. 67 ± 14.32μmol/mg.dw)亦高于对照组和高脂组，但无统计学差异；反映血管钙化程度的指标ALP活性在1型和2型糖尿病组（89. 46 ± 17.8 和 63.68 ±17. 83 U/mg. pr)亦明显高于对照组和高脂组（31. 22 ± 19. 42 和 31. 62 ± 15. 96 U/mg. pr) (P <0. 01 ,P <0. 05)； 1 型糖尿病组大鼠的股骨骨密度较其他 3 组均降低（0. 1898 ±0. 0263 vs 0. 224 ±0. 005,0. 2374 ±0. 0113,2431 ±0. 0115 g/cm2) (P<0.01)。结论 糖尿病大鼠主动脉钙含量和骨密度均有变化,提示糖尿病可引起体内钙磷代谢异常,其机制有待进一步探讨。 |
| 英文摘要: |
| Objective To observe the changes of serum calcium content and bone mineral density (BMD) in the diabetic rat model. Methods One-time intraperitoneal injection of high-dose streptozotocin ( STZ) or high fat and sugar feed and low-dose STZ injection was performed to establish type 1 or type 2 diabetic rat model. Thirty-two male Wistar rats were divided into 4 groups (8 in each group), the control group, high-fat group, type 1 diabetes group, and type 2 diabetes group. After 12 weeks, the aortic calcium content，alkaline phosphatase (ALP) activity, and osteocalcin (OC) content were determined. BMD of the femur and lumbar vertebra was detected. Results The blood calcium content in type 1 diabetes group (71. 67 ± 10. 61μmol/mg.dw) was higher than that in the control group (44 ± 15. 87μmol/mg. dw) and high-fat group (44. 8 ±9. 44 ) μmol/mg. dw,P<0. 05). The blood calcium content in type 2 diabetes group was higher than that in the control group and high-fat group, with no statistical difference. ALP activity in type 1 and type 2 diabetes groups (89.46 ± 17. 8 and 63. 68 ± 17. 83 U/mg. pr) was also higher than that in the control group and high-fat group (31. 22 ± 19. 42 and 31. 62 ± 15. 96 U/mg. pr, P <0. 01, P <0, 05).BMD of the femur in type 1 diabetes group was lower than that in the other groups (0. 1898±0, 0263 vs 0. 224 ±0. 005 and 0. 2374 ±0, 0113 and 2431 土 0.0115 g/cm2,. P < 0. 01 ). Conclusion The aortic calcium content and BMD change in diabetic rat models, indicating that diabetes can cause calcium phosphorus metabolism disorder in rats. The mechanism remains to be further discussed. |
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