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| 维生素D受体检测方法的研究进展 |
| Research progress in the vitamin D receptor assays |
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| DOI：10.3969/j.issn.1006.7108.2015.11.024 |
| 中文关键词: Vitamin D Research Institute, Shaanxi University of Technology, Hanzhong 723001，Shaanxi, China |
| 英文关键词:Vitamin D receptor Calcium and phosphorus metabolism Detection method Immunohistochemistry |
| 基金项目:陕西省重点科技创新团队（2012KTC-29);陕西省重点实验室计划项目（2011HBSCS003);陕西省教育厅专项科研计划项 目（12JS029) |
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| 中文摘要: |
| 维生素D不仅能够调节钙磷代谢,影响骨的生长，还能调节机体的免疫活性和细胞生长与分化。维生素D的功能是通过激活细胞核内的维生素D受体（vitamin D receptor, VDR)来实现的，因此，VDR是靶细胞响应维生素D反应的决定因素。VDR高表达的组织，如肠、肾脏、骨和甲状旁腺等，是维生素D的主要靶组织。过去40年中，为了测定VDR在机体各个组织中的分布，建立了多种检测方法。然而，这些测定方法的应用也产生了一些具有争议的结果，这可能是由检测方法的特异性不同导致的。近年来，本研究团队建立了一套特异性强、灵敏性高的免疫测定方法，并将其成功应用于测定组织中VDR。本文将对这些检测方法的的优点和各自的局限性进行讨论,并指出测定方法的选择是成功鉴定靶组织中VDR的关键。 |
| 英文摘要: |
| The major biological function of vitamin D is to regulate the metabolism of calcium and phosphate, and to modulate immune activity and cellular proliferation and differentiation. It is believed that vitamin D acts through binding to its nuclear receptor, vitamin D receptor (VDR). Therefore, the receptor is a determinant for a cell to respond to the vitamin D. The tissues containing high levels of VDR, such as intestine, kidney, bone and parathyroid, are known as the main targets of vitamin D. In the past 4 decades, several assays have been established for determining VDR in various tissues. However, the application of these methods also results in controversial results perhaps due to the varied specificity of each assay. We have recently established highly specific and sensitive immunoassays, which have been successfully used to determine VDR in the selected tissues. In this review, we discuss advantages and limitations of each assay and point out that the assay selection may be a key for the success in finding the receptor in target tissues. |
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